Efficacy and Safety of Novel Beta-Chitin Patches as Haemostat in Rat Vascular and Neurosurgical Model

Frontiers in Surgery

article

Peter-John Wormald¹  Ahad Sabab¹  Rajan Sundaresan Vediappan¹  John Finnie²  C. John McAdam³  Alistair Jukes¹  Sarah Vreugde¹ 

[1] Department of Surgery-Otorhinolaryngology, Head and Neck Surgery, University of Adelaide;Discipline of Anatomy and Pathology, Adelaide Medical School, University of Adelaide;Department of Chemistry, University of Otago

关键词: chitin;    p-GlcNAc;    haemostat;    haemostasis;    neurosurgery;    bio-technology;   

DOI  :  10.3389/fsurg.2022.830364

学科分类：社会科学、人文和艺术（综合）

来源: Frontiers

【 摘 要 】

Background Intraoperative hemorrhage is a major cause of poor post-operative outcome. Beta-chitin patch has previously been found to be an effective haemostat, but whether modifying the patch can improve its efficacy and safety, remains unknown. In this study, beta-chitin patches were modified using polyethylene oxide, Pluronic-F127 (Chi/F127), calcium (Chi/20%Ca), increased thickness (Chi/Thick) or polyphosphate (Chi/PP). Objective Using rat (Wistar Albino; 8–10 weeks old) vascular and neurosurgical models, this project investigated and compared the efficacy and safety of beta-chitin patches with gauze, Surgicel and FloSeal. Methods Ninety rats underwent a standardized femoral artery injury and were randomized to receive either beta-chitin patches, gauze, Surgicel or FloSeal. The bleeding time and total blood loss was measured. For the neurosurgical model, forty-four rats underwent a standardized cortical injury and randomization to a treatment group. Following a 48 h recovery period, their brains were collected for histopathological examination. Results The mean bleeding time with Chitin (120.8 s) and Chi/PP (117.3 s) was ~60 s lower than Chi/F127, Chi/20%Ca and Chi/Thick ( p < 0.05). Chitin and Chi/PP had a significantly lower bleeding time than FloSeal (174.2 s) ( p < 0.05), but not Surgicel (172.7 s). Gauze (400 s) had a significantly higher bleeding time compared to all other groups ( p < 0.05). There were no significant differences in the total blood loss between the groups. Histopathological examination of brains found no adverse inflammatory reaction to any of the haemostatic compounds. Conclusion Chi/PP had superior haemostatic efficacy compared to Surgicel and FloSeal, but not compared to non-modified beta-chitin patch. All of the haemostats were equally safe.

【 授权许可】

CC BY