Chondrogenically Tuned Expansion Enhances the Cartilaginous Matrix-Forming Capabilities of Primary, Adult, Leporine Chondrocytes

Cell Transplantation

Article

Jerry C. Hu¹  Daniel J. Huey¹  Kyriacos A. Athanasiou¹ 

[1] Department of Biomedical Engineering, University of California-Davis, Davis, CA, USA;

关键词: Cartilage;    Tissue engineering;    Serum free;    Chondrocyte;    Self-assembly;   

DOI  :  10.3727/096368912X657648

 received in 2011-06-22, accepted in 2012-04-25,  发布年份 2013

来源: Sage Journals

【 摘 要 】

When expanded through passage, chondrocytes lose their ability to produce high-quality cartilaginous matrix. This study attempts to improve the properties of constructs formed with expanded chondrocytes through alterations in the expansion protocol and the ratio of primary to expanded chondrocytes used to form cartilage constructs. A chondrogenically tuned expansion protocol provided similar monolayer growth rates as those obtained using serum-containing medium and enhanced cartilaginous properties of resultant constructs. Various ratios of primary to chondrogenically expanded chondrocytes were then self-assembled to form neocartilage. Biochemical analysis showed that constructs formed with only expanded cells had twice the GAG per wet weight and collagen II/collagen I ratio compared to constructs formed with primary chondrocytes. Biomechanically, compressive properties of constructs formed with only passaged cells matched the instantaneous modulus and exceeded the relaxation modulus of constructs formed with only primary cells. These counterintuitive results show that, by applying proper expansion and three-dimensional culture techniques, the cartilage-forming potential of adult chondrocytes expanded through passage can be enhanced over that of primary cells.

【 授权许可】

Unknown   
© 2013 Cognizant Comm. Corp.

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