Effect of Glucocorticoid and Melatonin on Immune Function of an Indian Tropical Bird, Perdicula Asiatica: An in Vivo and in Vitro Study

European Journal of Inflammation

Article

C. Haldar¹  S.S. Singh²  S.K. Yadav² 

[1] Professor C. Haldar, Pineal Research Laboratory, Department of Zoology, Banaras Hindu University, Varanasi - 221 005, India. Fax: ++91 542 2368174. e-mail: ;Pineal Research Laboratory, Department of Zoology, Banaras Hindu University, Varanasi, India;

关键词: dexamethasone;    melatonin;    trade-off;    splenocytes proliferation;    cytokines;    reproductive phases;   

DOI  :  10.1177/1721727X1000800206

 received in 2009-11-11, accepted in 2010-05-04,  发布年份 2010

来源: Sage Journals

【 摘 要 】

Inverse relationship between circulatory levels of glucocorticoids and melatonin has been reported for most of the vertebrates including human beings. We report the importance of glucocorticoid along with melatonin in avian immune regulation in an Indian tropical bird, Perdicula asiatica with in vivo and in vitro approach. Dexamethasone (30μg/bird/day) treatment of the male birds suppressed the immune activity as judged by the spleen activity (mass, anatomy), circulating total leukocyte, and lymphocyte count, blastogenic response, increased % apoptosis and cytokine (IL-2) production by splenocytes. Melatonin (25μg/100g B.wt./day) treatment increased the above-mentioned immune parameters whereas melatonin together with dexamethasone restored the suppressed immune parameters by dexamethasone to control level. In vitro melatonin (2.5 pM) supplementation to splenocyte cultures restored the dexamethasone (2 μM) suppressed splenocyte proliferation, % apoptosis and IL-2 production. Therefore, melatonin antagonized the suppressive effect of synthetic glucocorticoid on all immune parameters studied in vivo as well as in vitro. Furthermore, exogenous administration of dexamethasone and melatonin treatment altered the circulatory level of corticosterone and melatonin in an inverse manner. It is therefore, suggested that a hormonal trade-off between glucocorticoid and melatonin exists under in vivo and in vitro conditions, being involved in maintenance of the immune function of P. asiatica probably by involving cytokines i.e. IL-2 mediated pathway.

【 授权许可】

Unknown   
© 2010 SAGE Publications