Cell Transplantation | |
Forever Young: How to Control the Elongation, Differentiation, and Proliferation of Cells Using Nanotechnology | |
Article | |
J. Guo1  D. K. C. Tay1  Y. X. Liang1  K. F. So2  R. G. Ellis-Behnke3  W. Wu4  G. E. Schneider5  L. A. Teather5  | |
[1] Department of Anatomy, The University of Hong Kong Li Ka Shing Faculty of Medicine, Pokfulam, Hong Kong SAR, China;Department of Anatomy, The University of Hong Kong Li Ka Shing Faculty of Medicine, Pokfulam, Hong Kong SAR, China;State Key Lab for Brain & Cognitive Sciences, The University of Hong Kong Li Ka Shing Faculty of Medicine, Pokfulam, Hong Kong SAR, China;Research Centre of Heart, Brain, Hormone and Healthy Aging, The University of Hong Kong Li Ka Shing Faculty of Medicine, Pokfulam, Hong Kong SAR, China;Department of Anatomy, The University of Hong Kong Li Ka Shing Faculty of Medicine, Pokfulam, Hong Kong SAR, China;State Key Lab for Brain & Cognitive Sciences, The University of Hong Kong Li Ka Shing Faculty of Medicine, Pokfulam, Hong Kong SAR, China;Research Centre of Heart, Brain, Hormone and Healthy Aging, The University of Hong Kong Li Ka Shing Faculty of Medicine, Pokfulam, Hong Kong SAR, China;Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA;Department of Anatomy, The University of Hong Kong Li Ka Shing Faculty of Medicine, Pokfulam, Hong Kong SAR, China;State Key Lab for Brain & Cognitive Sciences, The University of Hong Kong Li Ka Shing Faculty of Medicine, Pokfulam, Hong Kong SAR, China;Research Centre of Heart, Brain, Hormone and Healthy Aging, The University of Hong Kong Li Ka Shing Faculty of Medicine, Pokfulam, Hong Kong SAR, China;Research Center of Reproduction, Development and Growth, The University of Hong Kong Li Ka Shing Faculty of Medicine, Pokfulam, Hong Kong SAR, China;Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA; | |
关键词: Nanotechnology; Stem cells; PC12; Schwann cells; Neural precursors; Brain; Spinal cord; Regeneration; Tissue engineering; Growth control; Self-assembling nanofiber scaffold (SAPNS); | |
DOI : 10.3727/096368909X471242 | |
来源: Sage Journals | |
【 摘 要 】
Within the emerging field of stem cells there is a need for an environment that can regulate cell activity, to slow down differentiation or proliferation, in vitro or in vivo while remaining invisible to the immune system. By creating a nanoenvironment surrounding PC12 cells, Schwann cells, and neural precursor cells (NPCs), we were able to control the proliferation, elongation, differentiation, and maturation in vitro. We extended the method, using self-assembling nanofiber scaffold (SAPNS), to living animals with implants in the brain and spinal cord. Here we show that when cells are placed in a defined system we can delay their proliferation, differentiation, and maturation depending on the density of the cell population, density of the matrix, and the local environment. A combination of SAPNS and young cells can be implanted into the central nervous system (CNS), eliminating the need for immunosuppressants.
【 授权许可】
Unknown
© 2009 Cognizant Comm. Corp.
【 预 览 】
Files | Size | Format | View |
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RO202212208951118ZK.pdf | 7479KB | download | |
Table 5. | 36KB | Table | download |
Table 6. | 35KB | Table | download |
Figure 2. | 178KB | Image | download |
Table 7. | 54KB | Table | download |
Table 8. | 52KB | Table | download |
Figure 5. | 103KB | Image | download |
Figure 6. | 469KB | Image | download |
Figure 1 | 986KB | Image | download |
【 图 表 】
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