Forever Young: How to Control the Elongation, Differentiation, and Proliferation of Cells Using Nanotechnology

Cell Transplantation

Article

J. Guo¹  D. K. C. Tay¹  Y. X. Liang¹  K. F. So²  R. G. Ellis-Behnke³  W. Wu⁴  G. E. Schneider⁵  L. A. Teather⁵ 

[1] Department of Anatomy, The University of Hong Kong Li Ka Shing Faculty of Medicine, Pokfulam, Hong Kong SAR, China;Department of Anatomy, The University of Hong Kong Li Ka Shing Faculty of Medicine, Pokfulam, Hong Kong SAR, China;State Key Lab for Brain & Cognitive Sciences, The University of Hong Kong Li Ka Shing Faculty of Medicine, Pokfulam, Hong Kong SAR, China;Research Centre of Heart, Brain, Hormone and Healthy Aging, The University of Hong Kong Li Ka Shing Faculty of Medicine, Pokfulam, Hong Kong SAR, China;Department of Anatomy, The University of Hong Kong Li Ka Shing Faculty of Medicine, Pokfulam, Hong Kong SAR, China;State Key Lab for Brain & Cognitive Sciences, The University of Hong Kong Li Ka Shing Faculty of Medicine, Pokfulam, Hong Kong SAR, China;Research Centre of Heart, Brain, Hormone and Healthy Aging, The University of Hong Kong Li Ka Shing Faculty of Medicine, Pokfulam, Hong Kong SAR, China;Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA;Department of Anatomy, The University of Hong Kong Li Ka Shing Faculty of Medicine, Pokfulam, Hong Kong SAR, China;State Key Lab for Brain & Cognitive Sciences, The University of Hong Kong Li Ka Shing Faculty of Medicine, Pokfulam, Hong Kong SAR, China;Research Centre of Heart, Brain, Hormone and Healthy Aging, The University of Hong Kong Li Ka Shing Faculty of Medicine, Pokfulam, Hong Kong SAR, China;Research Center of Reproduction, Development and Growth, The University of Hong Kong Li Ka Shing Faculty of Medicine, Pokfulam, Hong Kong SAR, China;Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA;

关键词: Nanotechnology;    Stem cells;    PC12;    Schwann cells;    Neural precursors;    Brain;    Spinal cord;    Regeneration;    Tissue engineering;    Growth control;    Self-assembling nanofiber scaffold (SAPNS);   

DOI  :  10.3727/096368909X471242

来源: Sage Journals

【 摘 要 】

Within the emerging field of stem cells there is a need for an environment that can regulate cell activity, to slow down differentiation or proliferation, in vitro or in vivo while remaining invisible to the immune system. By creating a nanoenvironment surrounding PC12 cells, Schwann cells, and neural precursor cells (NPCs), we were able to control the proliferation, elongation, differentiation, and maturation in vitro. We extended the method, using self-assembling nanofiber scaffold (SAPNS), to living animals with implants in the brain and spinal cord. Here we show that when cells are placed in a defined system we can delay their proliferation, differentiation, and maturation depending on the density of the cell population, density of the matrix, and the local environment. A combination of SAPNS and young cells can be implanted into the central nervous system (CNS), eliminating the need for immunosuppressants.

【 授权许可】

Unknown   
© 2009 Cognizant Comm. Corp.

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