Enabling Autologous Human Liver Regeneration with Differentiated Adipocyte Stem Cells

Cell Transplantation

Article

Gary Peltz¹  Manhong Wu¹  Dan Xu¹  Ming Zheng¹  Toshihiko Nishimura¹  Hua Su²  Noboru Sato²  Jeffrey Glenn³  Sara Michie⁴  Gordon Lee⁵ 

[1] Department of Anesthesia, Stanford University School of Medicine, Stanford, CA, USA;Department of Biochemistry, University of California-Riverside, Riverside, CA, USA;Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA;Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA;Department of Plastic Surgery, Stanford University School of Medicine, Stanford, CA, USA;

关键词: Liver regeneration;    Adipocyte stem cells;    Chimeric mice;    Induced hepatocyte-like cells;   

DOI  :  10.3727/096368913X673432

 received in 2013-07-25, accepted in 2013-09-03,  发布年份 2014

来源: Sage Journals

【 摘 要 】

We developed a novel method for differentiating adipocyte-derived stem cells (ASCs) into hepatocyte-like cells (iHeps). ASCs are cultured as spherical cellular aggregates and are then induced by culture in chemically defined media for a short time period to differentiate into spherical culture iHeps (SCi-Heps). SCi-Heps have many of the in vitro functional properties of mature hepatocytes, and they can stably reconstitute functioning human liver in vivo in a murine model system. Implantation studies demonstrate that SCi-Heps have a very low malignant potential. All human liver regenerative procedures, including ultrasound-guided direct liver implantation, are scalable and appropriate for human clinical use. These methods can be used to achieve the major promise of regenerative medicine. It may now be possible to regenerate human liver using autologous stem cells obtained from a readily accessible tissue.

【 授权许可】

Unknown   
© 2014 Cognizant Comm. Corp.

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