hUC-MSCs Exert a Neuroprotective Effect via Anti-apoptotic Mechanisms in a Neonatal HIE Rat Model

Cell Transplantation

Original Articles

Xianyao Wang¹  Huanxiang Zhang²  Jianwei Xu³  Lan Wang⁴  Zhanhui Feng⁵  Ying Xiong⁶  Lan Ye⁶ 

[1] Center for Tissue Engineering and Stem Cell Research, Guizhou Medical University, China;Department of Cell Biology, Medical College of Soochow University, China;Department of Cell Biology, Medical College of Soochow University, China;Center for Tissue Engineering and Stem Cell Research, Guizhou Medical University, China;Both the authors are co-first authors and contributed equally to this article.;Neurological Department, Affiliated Hospital of Guizhou Medical University, China;Neurological Department, Affiliated Hospital of Guizhou Medical University, China;Both the authors are co-first authors and contributed equally to this article.;School of Basic Medicine, Guizhou Medical University, China;

关键词: hUC-MSCs;    hypoxic-ischemic encephalopathy (HIE);    apoptosis;    caspase-3;    Beclin-2;   

DOI  :  10.1177/0963689719874769

 received in 2018-07-17, accepted in 2019-08-07,  发布年份 2019

来源: Sage Journals

【 摘 要 】

In this study, we investigated how human umbilical cord mesenchymal stem cells exerted a neuroprotective effect via antiapoptotic mechanisms in a neonatal hypoxic-ischemic encephalopathy rat model. A total of 78 10-day old (P10) rats were used. After human umbilical cord mesenchymal stem cells were collected from human umbilical cords and amplified in culture, they were administered to rat subjects 1 h after induced hypoxic-ischemic encephalopathy treatment. The short-term (48 h) and long-term (28 day) outcomes were evaluated after human umbilical cord mesenchymal stem cells treatment using neurobehavioral function assessment. Triphenyltetrazolium chloride monohydrate staining was performed at 48 h. Beclin-2 and caspase-3 levels were evaluated with Western blot and real time polymerase chain reaction at 48 h. Human umbilical cord mesenchymal stem cells were collected and administrated to hypoxic-ischemic encephalopathy pups by intracerebroventricular injection. Hypoxic-ischemic encephalopathy typically induced significant delay in development and caused impairment in both cognitive and motor functions in rat subjects. Human umbilical cord mesenchymal stem cells were shown to ameliorate hypoxic-ischemic encephalopathy-induced damage and improve both cognitive and motor functions. Although hypoxic-ischemic encephalopathy induced significant expression of caspase-3 and Beclin-2, human umbilical cord mesenchymal stem cells decreased the expression of both of them. Human umbilical cord mesenchymal stem cells may serve as a potential treatment to ameliorate brain injury in hypoxic-ischemic encephalopathy patients.

【 授权许可】

CC BY-NC   
© The Author(s) 2019

【 图 表 】

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