A Novel Strategy Incorporated the Power of Mesenchymal Stem Cells to Allografts for Segmental Bone Tissue Engineering

Cell Transplantation

Erratum

Zi Yin¹  Xiao Chen¹  Yang Zi Jiang¹  Hong Xin Cai²  Hong Wei Ouyang³  Hu Hu⁴  Xiao Hui Zou⁵ 

[1] Center for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University, China;Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, China;Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, China; Center for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University, China;Department of Pathology and Pathophysiology, School of Medicine, Zhejiang University, China;Women Hospital, School of Medicine, Zhejiang University, China;

关键词: Mesenchymal stem cells;    Bone allografts;    Tissue engineering;   

DOI  :  10.3727/09638910X539047

 received in 2008-03-28, accepted in 2008-11-24,  发布年份 2010

来源: Sage Journals

【 摘 要 】

Mesenchymal stem cells (MSCs) hold great promise for bone regeneration. However, the power of mesenchymal stem cells has not been applied to structural bone allografts in clinical practice. This study designed a new strategy to enhance the efficiency of allografts for segmental bone regeneration. Isolated MSCs were cultured to form a cell sheet. The MSC sheet was then wrapped onto structural allografts. The assembled structures were cultured in vitro to evaluate the differentiation potential of MSC sheet. The assembled structures were implanted subcutaneously into nude mice as well as into the segmental radius defect of rabbits to investigate the efficiency of MSC sheets to repopulate allografts for bone repair. MSC sheets, upon assembling on bone grafts, showed similar differentiation properties to the in situ periosteum in vitro. After implantation the MSC sheets accelerated the repopulation of bone grafts in nude mice. Moreover, MSC sheets induced thicker cortical bone formation and more efficient graft-to-bone end fusion at the segmental bone defects in rabbits. This study thus presented a novel, more efficient, and practical strategy for large weight-bearing bone reconstruction by using MSC sheets to deliver large number of MSCs to repopulate the bone allografts.

【 授权许可】

Unknown   
© 2010 Cognizant Comm. Corp.