Novel Cell Seeding System into a Porous Scaffold Using a Modified Low-Pressure Method to Enhance Cell Seeding Efficiency and Bone Formation

Cell Transplantation

Article

Makoto Takahashi¹  Ichiro Torigoe¹  Toshitaka Yoshii¹  Shigenori Kawabata¹  Shinichi Sotome¹  Akio Tsuchiya²  Kenichi Shinomiya³ 

[1] Graduate School, Tokyo Medical and Dental University, Tokyo, 113-8519, Japan;Graduate School, Tokyo Medical and Dental University, Tokyo, 113-8519, Japan; Molecular Destruction and Reconstruction of Tooth and Bone, Tokyo Medical and Dental University, Tokyo, 101-0062, Japan;Graduate School, Tokyo Medical and Dental University, Tokyo, 113-8519, Japan; Molecular Destruction and Reconstruction of Tooth and Bone, Tokyo Medical and Dental University, Tokyo, 101-0062, Japan; Hard Tissue Genome Research Center, Tokyo Medical and Dental University, Tokyo, 101-0062, Japan; Core to Core Program for Advanced Bone and Joint Science, Tokyo Medical and Dental University, Tokyo, 101-0062, Japan;

关键词: Tissue engineering;    Bone formation;    Porous scaffolds;    Bone marrow-derived stromal cells (BMSCs);    Cell seeding method;    Low pressure;   

DOI  :  10.3727/000000007783465109

 received in 2007-01-22, accepted in 2007-04-02,  发布年份 2007

来源: Sage Journals

【 摘 要 】

The efficient seeding of cells into porous scaffolds is important in bone tissue engineering techniques. To enhance efficiency, we modified the previously reported cell seeding techniques using low-pressure conditions. In this study, the effects of low pressure on bone marrow-derived stromal cells (BMSCs) of rats and the usefulness of the modified technique were assessed. There was no significant difference found in the proliferative and osteogenic capabilities among various low-pressure (50–760 mmHg, 1–10 min) conditions. To analyze the efficacies of the cell seeding techniques, BMSCs suspended in the plasma of rats were seeded into porous β-tricalcium phosphate (β-TCP) blocks by the following three procedures: 1) spontaneous penetration of cell suspension under atmospheric pressure (SP); 2) spontaneous penetration and subsequent low pressure treatment (SPSL), the conventional technique; and 3) spontaneous penetration under low pressure conditions (SPUL), the modified technique. Subsequently, these BMSCs/β-TCP composites were used for the analysis of cell seeding efficiency or in vivo bone formation capability. Both the number of BMSCs seeded into β-TCP blocks and the amount of bone formation of the SPUL group were significantly higher than those of the other groups. The SPUL method with a simple technique permits high cell seeding efficiency and is useful for bone tissue engineering using BMSCs and porous scaffolds.

【 授权许可】

Unknown   
© 2007 Cognizant Comm. Corp.