Cartilage Regeneration of Adipose-Derived Stem Cells in a Hybrid Scaffold from Fibrin-Modified PLGA

Cell Transplantation

Article

Hunyu Hu¹  Yinsong Wu¹  Haiqiang Wang¹  Jin Qi²  Lianfu Deng²  Yiyong Wei² 

[1] Institute of Orthopaedics and Traumatology, Xijing Hospital, The Fourth Military Medical University, Xi'an, People's Republic of China;Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, People's Republic of China;

关键词: Cartilage regeneration;    Adipose;    Stem cells;    PLGA;    Fibrin;    Scaffold;   

DOI  :  10.3727/096368909788341261

 received in 2008-02-28, accepted in 2008-09-12,  发布年份 2009

来源: Sage Journals

【 摘 要 】

Adipose-derived stem cells (ASCs) appear to be a useful stem cell population, which has been shown to possess multipotentiality. The aim of this study was to evaluate the utility of ASCs in tissue-engineered cartilage using a hybrid scaffold from fibrin-modified PLGA scaffold. ASCs were isolated from rabbit adipose tissue. The PLGA scaffold was prepared by low-temperature deposition technology and the hybrid scaffold was fabricated by a freeze-drying method. When ASCs were seeded onto fibrin-modified PLGA scaffold in vitro, enhanced cellular viability was observed compared to unmodified PLGA scaffold. The analysis of proteoglycan and collagen II revealed that fibrin-modified scaffold succeeded in inducing ASCs to differentiate into chondrocytes in vitro. A preliminary study on cartilage regeneration was also performed in vivo. Observation of histology and immunoblotting demonstrated that ASCs containing the hybrid scaffold promoted cartilage regeneration in the defects of articular cartilage much better than other groups. These results indicated that ASCs containing the hybrid scaffold are a more effective way to potentially enhance articular cartilage regeneration.

【 授权许可】

Unknown   
© 2009 Cognizant Comm. Corp.

【 图 表 】

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