Decellularization and Recellularization Technologies in Tissue Engineering

Cell Transplantation

Review

Yu-Chi Wang¹  Shih-Ping Liu²  Shyh-Jer Huang³  Chang-Hai Tsai⁴  Ton-Ru Shih⁵  Jiun-Huei Sung⁵  Jing-Rong Wei⁵  Hsin-Lien Lin⁵  Zih-Wan Wang⁵  Yue-Mi Chen⁵  Chia-Hui Lu⁵  Ru-Huei Fu⁶  Woei-Cherng Shyu⁶  Shinn-Zong Lin⁷ 

[1] Biomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, Hsinchu, Taiwan;Center for Neuropsychiatry, China Medical University Hospital, Taichung, Taiwan;Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan;Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung, Taiwan;Department of Pediatrics, China Medical University, Taichung, Taiwan;Department of Healthcare Administration, Asia University, Taichung, Taiwan;Graduate Institute of Immunology, China Medical University, Taichung, Taiwan;Graduate Institute of Immunology, China Medical University, Taichung, Taiwan;Center for Neuropsychiatry, China Medical University Hospital, Taichung, Taiwan;Graduate Institute of Immunology, China Medical University, Taichung, Taiwan;Center for Neuropsychiatry, China Medical University Hospital, Taichung, Taiwan;Department of Neurosurgery, China Medical University Beigang Hospital, Yunlin, Taiwan;Department of Neurosurgery, Tainan Municipal An-Nan Hospital, China Medical University, Tainan, Taiwan;

关键词: Biomaterial;    Decellularization;    Extracellular matrix (ECM);    Organ bioengineering and regeneration;    Recellularization;   

DOI  :  10.3727/096368914X678382

 received in 2013-10-30, accepted in 2014-01-31,  发布年份 2014

来源: Sage Journals

【 摘 要 】

Decellularization is the process by which cells are discharged from tissues/organs, but all of the essential cues for cell preservation and homeostasis are retained in a three-dimensional structure of the organ and its extracellular matrix components. During tissue decellularization, maintenance of the native ultrastructure and composition of the extracellular matrix (ECM) is extremely acceptable. For recellularization, the scaffold/matrix is seeded with cells, the final goal being to form a practical organ. In this review, we focus on the biological properties of the ECM that remains when a variety of decellularization methods are used, comparing recellularization technologies, including bioreactor expansion for perfusion-based bioartificial organs, and we discuss cell sources. In the future, decellularization–recellularization procedures may solve the problem of organ assembly on demand.

【 授权许可】

Unknown   
© 2014 Cognizant Comm. Corp.