Cryopreservation of Human Pancreatic Islets from Non-Heart-Beating Donors Using Hydroxyethyl Starch and Dimethyl Sulfoxide as Cryoprotectants

Cell Transplantation

Article

Akiko Suzuki¹  Mariko Miyazaki¹  Michihiro Maruyama²  Naotake Akutsu²  Kazunori Ohtsuki²  Kenichi Saigo²  Chikara Iwashita²  Takashi Kenmochi³  Takehide Asano⁴ 

[1] Clinical Research Center, Chiba-East National Hospital, National Hospital Organization, Chiba, Japan;Department of Surgery, Chiba-East National Hospital, National Hospital Organization, Chiba, Japan;Department of Surgery, Chiba-East National Hospital, National Hospital Organization, Chiba, Japan;Clinical Research Center, Chiba-East National Hospital, National Hospital Organization, Chiba, Japan;Department of Surgical Oncology, Chiba Cancer Center, Chiba, Japan;

关键词: Cryopreservation;    Human islets;    Hydroxyethyl starch;    Islet transplantation;   

DOI  :  10.3727/000000008783907026

 received in 2007-02-05, accepted in 2007-09-30,  发布年份 2008

来源: Sage Journals

【 摘 要 】

Although widely used, DMSO is toxic for pancreatic islets. We combined hydroxyethyl starch (HES) with DMSO to simplify the procedure of freezing and thawing, and to decrease the toxicity of DMSO. A preclinical study was performed using islets from beagle dogs. After storage for 4 weeks, the islets were thawed and examined. The islet structure was well maintained after thawing. Although the number of the islets decreased to 71.2 ± 20.1%, the function of the islets was evaluated by static incubation after thawing and showed a 1.80 ± 0.78 stimulation index. We have introduced this technique for the cryopreservation of human islets from non-heart-beating donors. Twelve cases of human islet cryopreservation were performed. The sample tube of each human cryopreservation was thawed to evaluate the morphology, contamination, and endocrine function. Although fragmentation was observed in five samples (41.6%), the other seven (58.4%) showed a normal structure when evaluated by microscopic and electron microscopic study. The stimulation index (SI) of static incubation deteriorated from 3.37 ± 3.02 to 1.34 ± 0.28 after thawing. We divided the thawed islets into two groups: group 1 (n = 8), SI >1.2; group 2 (n = 4), SI <1.2. The group 1 islets showed a higher rate of normal structure (87%) than did group 2 (25%). Moreover, the SI before cryopreservation was 4.01 ± 3.57 in group 1, which was higher than the SI of 2.11 ± 0.72 in group 2. Based on the good results from the preclinical study using a large-animal model, this method was introduced for clinical application. Even from the pancreata of non-heart-beating donors, a successful islet cryopreservation was achieved. However, the isolated islets with poor function should not be cryopreserved for transplantation.

【 授权许可】

Unknown   
© 2008 Cognizant Comm. Corp.