Therapeutic Effects of Mouse Adipose-Derived Stem Cells and Losartan in the Skeletal Muscle of Injured Mdx Mice

Cell Transplantation

Article

Choong-Yong Kim¹  Jin-Kyu Park²  Eun-Mi Lee³  Meeyul Hwang³  Ah-Young Kim³  Myeong-Mi Lee³  Kyu-Shik Jeong³  Eun-Joo Lee³  Shin-Yoon Kim⁴ 

[1] Animal Center, Daegu-Gueongbuk Medical Innovation Foundation, Daegu City, Republic of Korea;College of Veterinary Medicine, Kyungpook National University, Daegu City, Republic of Korea;College of Veterinary Medicine, Kyungpook National University, Daegu City, Republic of Korea; Stem Cell Therapeutic Research Institute, Kyungpook National University, Daegu City, Republic of Korea;Department of Orthopedic Surgery, Kyungpook National University School of Medicine, Deagu City, Republic of Korea; Skeletal Diseases Genome Research Center, Kyungpook National University and Hospital, Deagu City, Republic of Korea;

关键词: Adipose stem cells;    Losartan;    Stem cell microenvironment;    Muscular dystrophy;    Skeletal muscle regeneration;   

DOI  :  10.3727/096368914X678599

 received in 2012-01-20, accepted in 2014-01-27,  发布年份 2015

来源: Sage Journals

【 摘 要 】

Duchenne muscular dystrophy (DMD) is an X-linked genetic disorder caused by mutations in the dystrophin gene. Adipose-derived stem cells (ASCs) are an attractive source of cells for stem cell therapy. Losartan has been reported to improve ASC transplantation in injured mouse muscles. In the present study, we investigated whether the combined treatment of losartan and ASCs in the injured muscles of mdx mice improves regeneration. The combined treatment of ASCs and losartan remarkably improved muscle regeneration and induced muscle hypertrophy. In addition, ASCs and losartan treatment downregulated transforming growth factor-β and inhibited muscle fibrosis. We observed cells coexpressing green fluorescent protein (GFP) and dystrophin in the muscle samples of mice transplanted with GFP-positive ASCs. In the coculture in vitro experiment, we also observed that the GFP ASCs differentiated into dystrophin-expressing myotubes. The present study shows that the combination of transplanted ASCs and treatment with losartan ameliorated muscle fibrosis and improved muscle regeneration in injured mdx mice. Thus, we suggest that combined treatment with losartan and ASCs could help to improve muscle regeneration in the muscles of injured patients, including DMD patients.

【 授权许可】

Unknown   
© 2015 Cognizant Comm. Corp.

【 图 表 】

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