Cell Transplantation | |
Therapeutic Effects of Mouse Adipose-Derived Stem Cells and Losartan in the Skeletal Muscle of Injured Mdx Mice | |
Article | |
Choong-Yong Kim1  Jin-Kyu Park2  Eun-Mi Lee3  Meeyul Hwang3  Ah-Young Kim3  Myeong-Mi Lee3  Kyu-Shik Jeong3  Eun-Joo Lee3  Shin-Yoon Kim4  | |
[1] Animal Center, Daegu-Gueongbuk Medical Innovation Foundation, Daegu City, Republic of Korea;College of Veterinary Medicine, Kyungpook National University, Daegu City, Republic of Korea;College of Veterinary Medicine, Kyungpook National University, Daegu City, Republic of Korea; Stem Cell Therapeutic Research Institute, Kyungpook National University, Daegu City, Republic of Korea;Department of Orthopedic Surgery, Kyungpook National University School of Medicine, Deagu City, Republic of Korea; Skeletal Diseases Genome Research Center, Kyungpook National University and Hospital, Deagu City, Republic of Korea; | |
关键词: Adipose stem cells; Losartan; Stem cell microenvironment; Muscular dystrophy; Skeletal muscle regeneration; | |
DOI : 10.3727/096368914X678599 | |
received in 2012-01-20, accepted in 2014-01-27, 发布年份 2015 | |
来源: Sage Journals | |
【 摘 要 】
Duchenne muscular dystrophy (DMD) is an X-linked genetic disorder caused by mutations in the dystrophin gene. Adipose-derived stem cells (ASCs) are an attractive source of cells for stem cell therapy. Losartan has been reported to improve ASC transplantation in injured mouse muscles. In the present study, we investigated whether the combined treatment of losartan and ASCs in the injured muscles of mdx mice improves regeneration. The combined treatment of ASCs and losartan remarkably improved muscle regeneration and induced muscle hypertrophy. In addition, ASCs and losartan treatment downregulated transforming growth factor-β and inhibited muscle fibrosis. We observed cells coexpressing green fluorescent protein (GFP) and dystrophin in the muscle samples of mice transplanted with GFP-positive ASCs. In the coculture in vitro experiment, we also observed that the GFP ASCs differentiated into dystrophin-expressing myotubes. The present study shows that the combination of transplanted ASCs and treatment with losartan ameliorated muscle fibrosis and improved muscle regeneration in injured mdx mice. Thus, we suggest that combined treatment with losartan and ASCs could help to improve muscle regeneration in the muscles of injured patients, including DMD patients.
【 授权许可】
Unknown
© 2015 Cognizant Comm. Corp.
【 预 览 】
Files | Size | Format | View |
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RO202212205748283ZK.pdf | 4177KB | download | |
Figure 11. | 59KB | Image | download |
Table 1. | 150KB | Table | download |
Figure 12. | 894KB | Image | download |
Table 2. | 109KB | Table | download |
Table 3. | 371KB | Table | download |
Figure 2 | 26KB | Image | download |
Table 4. | 477KB | Table | download |
【 图 表 】
Figure 2
Figure 12.
Figure 11.
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