期刊论文详细信息
Breast Cancer: Basic and Clinical Research
Formin Proteins FHOD1 and INF2 in Triple-Negative Breast Cancer: Association With Basal Markers and Functional Activities
Original Research
Samu Kurki1  Naziha Mansuri2  Maria Gardberg3  Heli Repo3  Pauliina Kronqvist3  Vanina D Heuser4  Olli Carpén5  Jasper Mogg6 
[1] Auria Biobank, Turku University Hospital and University of Turku, Turku, Finland;Institute of Biomedicine, University of Turku, Turku, Finland;Institute of Biomedicine, University of Turku, Turku, Finland;Department of Pathology, Turku University Hospital, Turku, Finland;Institute of Biomedicine, University of Turku, Turku, Finland;Department of Pathology, Turku University Hospital, Turku, Finland;MediCity Research Laboratory, University of Turku, Turku, Finland;Institute of Biomedicine, University of Turku, Turku, Finland;Department of Pathology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland;Northern Institute for Cancer Research, Newcastle University, Newcastle-upon-Tyne, UK;
关键词: Formin;    FHOD1;    INF2;    cytoskeleton;    actin;    triple-negative breast cancer;    basal-like breast cancer;    BT-549;    MDA-MB-231;   
DOI  :  10.1177/1178223418792247
 received in 2018-04-04, accepted in 2018-07-11,  发布年份 2018
来源: Sage Journals
PDF
【 摘 要 】

Basal-like breast cancer is an aggressive form of breast cancer with limited treatment options. The subgroup can be identified immunohistochemically, by lack of hormone receptor expression combined with expression of basal markers such as CK5/6 and/or epidermal growth factor receptor (EGFR). In vitro, several regulators of the actin cytoskeleton are essential for efficient invasion of basal-like breast cancer cell lines. Whether these proteins are expressed in vivo determines the applicability of these findings in clinical settings. The actin-regulating formin protein FHOD1 participates in invasion of the triple-negative breast cancer cell line MDA-MB-231. Here, we measure the expression of FHOD1 protein in clinical triple-negative breast cancers by using immunohistochemistry and further characterize the expression of another formin protein, INF2. We report that basal-like breast cancers frequently overexpress formin proteins FHOD1 and INF2. In cell studies using basal-like breast cancer cell lines, we show that knockdown of FHOD1 or INF2 interferes with very similar processes: maintenance of cell shape, migration, invasion, and proliferation. Inhibition of EGFR, PI3K, or mitogen-activated protein kinase activity does not alter the expression of FHOD1 and INF2 in these cell lines. We conclude that the experimental studies on these formins have implications in the clinical behavior of basal-like breast cancer.

【 授权许可】

CC BY-NC   
© The Author(s) 2018

【 预 览 】
附件列表
Files Size Format View
RO202212205540035ZK.pdf 1436KB PDF download
Table 1. 691KB Table download
Table 1. 76KB Table download
Figure 3. 1048KB Image download
Figure 4 27KB Image download
photo 1. 34KB Image download
Figure 1. 420KB Image download
【 图 表 】

Figure 1.

photo 1.

Figure 4

Figure 3.

【 参考文献 】
  • [1]
  • [2]
  • [3]
  • [4]
  • [5]
  • [6]
  • [7]
  • [8]
  • [9]
  • [10]
  • [11]
  • [12]
  • [13]
  • [14]
  • [15]
  • [16]
  • [17]
  • [18]
  • [19]
  • [20]
  • [21]
  • [22]
  • [23]
  • [24]
  • [25]
  • [26]
  • [27]
  • [28]
  • [29]
  • [30]
  • [31]
  • [32]
  • [33]
  • [34]
  • [35]
  • [36]
  • [37]
  文献评价指标  
  下载次数:20次 浏览次数:2次