Neural Stem/Progenitor Cells Transplanted to the Hypoglossal Nucleus Integrates with the Host CNS in Adult Rats and Promotes Motor Neuron Survival

Cell Transplantation

Article

Michael Fagerlund¹  Nasren Jaff¹  Mikael Svensson¹  Lou Brundin¹  Cynthia Pérez Estrada¹ 

[1] Department of Clinical Neuroscience and Departments of Neurosurgery and Neurology, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden;

关键词: Brain stem;    Neural progenitor cells;    Nerve injury;    Motor neurons;    Transplantation;    Integration;   

DOI  :  10.3727/096368911X612459

 received in 2010-08-30, accepted in 2011-04-15,  发布年份 2012

来源: Sage Journals

【 摘 要 】

Transplantation of neural stem cells and the mobilization of endogenous neuronal precursors in the adult brain have been proposed as therapeutic strategies for central nervous system disorders and injuries. The aim of the present study was to investigate the possible survival and integration of grafted neural progenitor cells (NPCs) from the subventricular zone (SVZ) in a hypoglossal nerve avulsion model with substantial neuronal loss. Adult neural progenitor cells (NPCs) from the subventricular zone (SVZ) were cultured from inbred transgenic eGFP Lewis rats and transplanted to the hypoglossal nucleus of inbred Lewis rat from the same family but that were not carrying the eGFP strain after avulsion of the hypoglossal nerve. Grafted cells survived in the host more than 3 months and differentiated into neurons [βIII tubulin (Tuj-1 staining)] with fine axon- and dendrite-like processes as well as astrocytes (GFAP) and oligodendrocytes (O4) with typical morphology. Staining for synaptic structures (synaptophysin and bassoon) indicated integration of differentiated cells from the graft with the host CNS. Furthermore, transplantation of NPCs increased the number of surviving motoneurons in the hypoglossal nucleus after nerve avulsion that, if untreated, result in substantial neuronal death. The NPCs used in this study expressed VEGF in vitro as well as in vivo following transplantation that may mediate the rescue effect of the axotomized motoneurons.

【 授权许可】

Unknown   
© 2012 Cognizant Comm. Corp.

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