Plasma as a Scaffold for Regeneration of Neural Precursor Cells after Transplantation into Rats with Spinal Cord Injury

Cell Transplantation

Article

Noboru Suzuki¹  Masaya Nakamura²  Hideyuki Okano³  Yukie Tokura⁴  Rie Igarashi⁴  Yuki Ohta⁴  Mitsuko Takenaga⁴  Akemi Hamaguchi⁴ 

[1] Department of Immunology and Medicine, St. Marianna University School of Medicine, Kawasaki 216–8512, Japan;Department of Orthopaedic Surgery, Keio University School of Medicine, Tokyo 160–8582, Japan;Department of Physiology, Keio University School of Medicine, Tokyo 160–8582, Japan;Institute of Medical Science, St. Marianna University School of Medicine, Kawasaki 216–8512, Japan;

关键词: Neural precursor cell;    Plasma;    Serum;    BBB score;    Spinal cord injury;   

DOI  :  10.3727/000000007783464434

 received in 2006-05-02, accepted in 2006-09-08,  发布年份 2007

来源: Sage Journals

【 摘 要 】

The present study investigated whether plasma could be useful as a scaffold for cell transplantation in rats with spinal cord injury (SCI). Transplantation of cells with plasma promoted the recovery of SCI-induced motor dysfunction. Immunohistochemical analysis revealed that the grafted cells had differentiated into the neural lineage. When dissociated neural precursor cells were cultured with plasma, extensive neurite outgrowth was observed along with increased expression of p35 and NF68. Neural markers were also expressed by the cultured cells. Culture with plasma reduced thymidine incorporation, but promoted cell growth and increased the RNA contents. These findings suggest that the cells underwent differentiation into neurons in the presence of plasma. In conclusion, plasma could be a promising scaffold for cell transplantation therapy.

【 授权许可】

Unknown   
© 2007 Cognizant Comm. Corp.