Subcultured Odontogenic Epithelial Cells in Combination with Dental Mesenchymal Cells Produce Enamel–Dentin-Like Complex Structures

Cell Transplantation

Article

M. Ueda¹  K. I. Hata²  Y. Shinohara³  M. J. Honda³ 

[1] Department of Oral and Maxillofacial Surgery, Nagoya University Postgraduate School of Medicine, Aichi 466-8550, Japan;Japan Tissue Engineering Co. Ltd, Aichi 443-0022, Japan;Tooth Regeneration, Division of Stem Cell Engineering, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan;

关键词: Ameloblast;    Enamel–dentin complex;    Odontogenic epithelial cells;    Subculture;    Tissue engineering;   

DOI  :  10.3727/000000007783465208

 received in 2007-02-06, accepted in 2007-06-05,  发布年份 2007

来源: Sage Journals

【 摘 要 】

We showed in a previous study that odontogenic epithelial cells can be selectively cultured from the enamel organ in serum-free medium and expanded using feeder layers of 3T3-J2 cells. The subcultured odontogenic epithelial cells retain the capacity for ameloblast-related gene expression, as shown by semiquantitative RT-PCR. The purpose of the present study was to evaluate the potential of subcultured odontogenic epithelial cells to form tooth structures in cell–polymer constructs maintained in vivo. Enamel organs from 6-month-old porcine third molars were dissociated into single odontogenic epithelial cells and subcultured on feeder layers of 3T3-J2 cells. Amelogenin expression was detected in the subcultured odontogenic epithelial cells by immunostaining and Western blotting. The subcultured odontogenic epithelial cells were seeded onto collagen sponge scaffolds in combination with fresh dental mesenchymal cells, and transplanted into athymic rats. After 4 weeks, enamel–dentin-like complex structures were present in the implanted constructs. These results show that our culture system produced differentiating ameloblast-like cells that were able to secrete amelogenin proteins and form enamel-like tissues in vivo. This application of the subculturing technique provides a foundation for further tooth-tissue engineering and for improving our understanding of ameloblast biology.

【 授权许可】

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© 2007 Cognizant Comm. Corp.