Transplants of Encapsulated Rat Choroid Plexus Cells Exert Neuroprotection in a Rodent Model of Huntington's Disease

Cell Transplantation

Article

Dwaine F. Emerich¹  Steven J. M. Skinner²  Marilyn Geaney²  Cesario V. Borlongan³  Christopher G. Thanos⁴ 

[1] LCT BioPharma, Inc., Providence, RI, USA;Living Cell Technologies Limited, Auckland, New Zealand;Neurology/Institute of Molecular Medicine and Genetics/School of Graduate Studies, Medical College of Georgia, Augusta, GA, USA;Research and Affiliations Service Line, Augusta VAMC, Augusta, GA, USA;Research and Affiliations Service Line, Augusta VAMC, Augusta, GA, USA;

关键词: Choroid plexus;    Transplant;    Huntington's disease;    Encapsulation;    Alginate;   

DOI  :  10.3727/000000007783472426

 received in 2007-07-21, accepted in 2007-10-14,  发布年份 2007

来源: Sage Journals

【 摘 要 】

Choroid plexus (CP) epithelial cells secrete several neurotrophic factors and have been used in transplantation studies designed to impart neuroprotection against central nervous system (CNS) trauma. In the present study, CP was isolated from adult rats, encapsulated within alginate microcapsules, and transplanted unilaterally into the rat striatum. Three days later, unilateral injections of quinolinic acid (QA; 225 nmol) were made into the ipsilateral striatum to mimic the pathology observed in Huntington's disease (HD). After surgery, animals were tested for motor function using the placement test. Rats receiving CP transplants were significantly less impaired on this test. Nissl-stained sections demonstrated that CP transplants significantly reduced the volume of the striatal lesion produced by QA. Quantitative analysis of striatal neurons further demonstrated that choline acetyltransferase-immunoreactive, but not diaphorase-positive, neurons were protected by CP transplants. These data demonstrate that transplanted CP cells can be used to protect striatal neurons from excitotoxic damage and that the pattern of neuroprotection varies across specific neuronal populations.

【 授权许可】

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© 2007 Cognizant Comm. Corp.