Bone-Forming Capacity and Biodistribution of Bone Marrow-Derived Stromal Cells Directly Loaded into Scaffolds: A Novel and Easy Approach for Clinical Application of Bone Regeneration

Cell Transplantation

Article

Philippe Bierling¹  Angélique Lebouvier²  Julie Léotot²  Nathalie Chevallier²  Hélène Rouard³  Philippe Hernigou⁴ 

[1] Etablissement Français du Sang d'Ile-de-France, Unité d'Ingénierie et de Thérapie Cellulaire, Créteil, France;INSERM UMR955, Paris-Est University, Créteil, France;Université Paris-Est Créteil, Faculté de médecine, Laboratoire de “Bioingénierie Cellulaire, Tissulaire et Sanguine,” Créteil, France;Etablissement Français du Sang d'Ile-de-France, Unité d'Ingénierie et de Thérapie Cellulaire, Créteil, France;Université Paris-Est Créteil, Faculté de médecine, Laboratoire de “Bioingénierie Cellulaire, Tissulaire et Sanguine,” Créteil, France;Etablissement Français du Sang d'Ile-de-France, Unité d'Ingénierie et de Thérapie Cellulaire, Créteil, France;AP-HP Hôpital Henri-Mondor – A. Chenevier, Service Hospitalier, Créteil, France;Université Paris-Est Créteil, Faculté de médecine, Laboratoire de “Bioingénierie Cellulaire, Tissulaire et Sanguine,” Créteil, France;Service de Chirurgie Orthopédique et Traumatologique, AP-HP Hôpital Henri-Mondor, Créteil, France;

关键词: Bone marrow-derived stromal cell transplantation;    Cell seeding;    Cell density;    Biodistribution;    Bone tissue engineering;   

DOI  :  10.3727/096368914X685276

 received in 2014-05-07, accepted in 2014-10-21,  发布年份 2015

来源: Sage Journals

【 摘 要 】

In the context of clinical applications of bone regeneration, cell seeding into scaffolds needs to be safe and easy. Moreover, cell density also plays a crucial role in the development of efficient bone tissue engineering constructs. The aim of this study was to develop and evaluate a simple and rapid cell seeding procedure on hydroxyapatite/ β-tricalcium phosphate (HA/βTCP), as well as define optimal cell density and control the biodistribution of grafted cells. To this end, human bone marrow-derived stromal cells (hBMSCs) were seeded on HA/βTCP scaffolds, and we have compared bone formation using an ectopic model. Our results demonstrated a significantly higher bone-forming capacity of hBMSCs directly loaded on HA/βTCP during surgery compared to hBMSCs preseeded for 7 days in vitro on HA/βTCP before ectopic implantation. The extent of new bone formation increases with increasing hBMSC densities quantitatively, qualitatively, and in frequency. Also, this study showed that grafted hBMSCs remained confined to the implantation site and did not spread toward other tissues, such as liver, spleen, lungs, heart, and kidneys. In conclusion, direct cell loading into a scaffold during surgery is more efficient for bone regeneration, as well as quick and safe. Therefore direct cell loading is suitable for clinical requirements and cell production control, making it a promising approach for orthopedic applications. Moreover, our results have provided evidence that the formation of a mature bone organ containing hematopoietic islets needs a sufficiently high local density of grafted hBMSCs, which should guide the optimal dose of cells for clinical use.

【 授权许可】

Unknown   
© 2015 Cognizant Comm. Corp.

【 图 表 】

Figure 5.