A Simple Breeding Protocol for the Procurement of Accurately Staged Rat Donor Embryos for Neural Transplantation

Cell Transplantation

Article

S. B. Dunnett¹  E. M. Torres¹  A. L. Baird¹  U. M. Weyrauch¹ 

[1] Department of Biosciences, Cardiff University, Cardiff, UK;

关键词: Rat breeding;    Cell transplantation;    Estrus cycle;    Embryo staging;    Donor age;   

DOI  :  10.3727/096368909788809802

 received in 2008-07-03, accepted in 2008-11-12,  发布年份 2009

来源: Sage Journals

【 摘 要 】

Obtaining accurately staged rat embryos can be difficult because of the variety of breeding protocols employed and because precise staging cannot be confirmed until excision of the embryos from the dam. The detection of estrus, pairing of animals, and confirmation of pregnancies is generally left to commercial suppliers, as in-house breeding can be laborious and unpredictable. Here we describe a simple, reliable in-house breeding protocol for the generation of accurately staged embryos as assessed by measurements of average crown to rump length (CRL).

【 授权许可】

Unknown   
© 2009 Cognizant Comm. Corp.

【 图 表 】

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