β-Catenin Accumulation is Associated with Increased Expression of Nanog Protein and Predicts Maintenance of MSC Self-Renewal

Cell Transplantation

Article

Hyun-Je Kim¹  Sang-Jin Yu²  Soung-Hoo Jeon³  Chung-Gyu Park³  Eui Seok Lee⁴  Su Jin Cho⁵ 

[1] Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul, Republic of Korea;Department of Biomedical Sciences, Seoul National University Graduate School, Seoul, Republic of Korea;Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul, Republic of Korea;FOS Clinic, SM Tower (3rd Floor), 334 Gangnam-Daero, Gangnam-Gu, Seoul 135-936, Republic of Korea.;Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul, Republic of Korea;Institute for Endemic Disease, Medical Research Center, Seoul National University College of Medicine, Seoul, Republic of Korea;Translational Xenotransplantation Research Center, Seoul National University College of Medicine, Seoul, Republic of Korea;Department of Oral and Maxillofacial Surgery, College of Medicine, Korea University, Guro Hospital, Seoul, Republic of Korea;Department of Pediatrics, Ewha Womans University School of Medicine, Seoul, Republic of Korea;

关键词: Human mesenchymal stem cells (hMSCs);    Self-renewing cells;    Epidermal growth factor (EGF);    β-Catenin;   

DOI  :  10.3727/096368916X693040

 received in 2015-12-29, accepted in 2016-12-07,  发布年份 2017

来源: Sage Journals

【 摘 要 】

Human mesenchymal stem cells (hMSCs) are self-renewing cells with the ability to differentiate into organized, functional network of cells. Recent studies have revealed that activation of the Wnt/β-catenin pathway by a glycogen synthase kinase (GSK)-3-specific pharmacological inhibitor, Bio, results in the maintenance of self-renewal in both mouse and human ES cells. The molecular mechanism behind the maintenance of hMSCs by these factors, however, is not fully understood. We found that rEGF enhances the level of β-catenin, a component of the Wnt/β-catenin signaling pathway. Furthermore, it was found that β-catenin upregulates Nanog. EGF activates the β-catenin pathway via the Ras protein and also increased the Nanog protein and gene expression levels 2 h after rEGF treatment. These results suggest that adding EGF can enhance β-catenin and Nanog expression in MSCs and facilitate EGF-mediated maintenance of MSC self-renewal. EGF was shown to augment MSC proliferation while preserving early progenitors within MSC population and thus did not induce differentiation. Thus, EGF not only can be used to expand MSC in vitro but also be utilized to autologous transplantation of MSCs in vivo.

【 授权许可】

Unknown   
© 2017 SAGE Publications Inc

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