Multilineage Potential of Side Population Cells from Human Amnion Mesenchymal Layer

Cell Transplantation

Article

T. Yakuwa¹  K. Sato¹  N. Sakuragawa²  K. Sasaki²  A. Kikuchi²  M. Kobayashi²  I. Kamo²  Y. Yokoyama³ 

[1] Department of Applied Life Science, Graduate School of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan;Department of Regenerative Medicine, School of Allied Health Sciences, Kitasato University, Nishi-hashimoto, Kanagawa, Japan;Stem Cell Processing, Inc., Nishi-hashimoto, Kanagawa, Japan;

关键词: Stem cells;    Amnion mesenchymal cells;    Side population cells;    Multilineage potential;    Mesenchymal cells;   

DOI  :  10.3727/096368908784153904

 received in 2007-07-23, accepted in 2007-08-27,  发布年份 2008

来源: Sage Journals

【 摘 要 】

Side population (SP) cells were isolated by FACS from a human amnion mesenchymal cell (AMC) layer soon after enzyme treatment. The yield of SP cells from AMC layer (AMC-SP cells) was about 0.1–0.2%. AMC-SP cells grew well with cell doublings of 40–80 days of culture. FACS profiles and immunocytostaining showed that AMC-SP cells were composed of two different cells immunologically: HLA I–/II- and HLA I+/II–. Oct-3/4 was detected in the nucleus of AMC-SP cells, when the culture was examined at the third, sixth, and 10th passages. RT-PCR showed that AMC-SP cells expressed the Oct-4, Sox-2, and Rex-1 genes. Immunocytochemistry revealed that all AMC-SP cells were vimentin+, CK19+, and nestin+. In addition, flow cytometry analysis showed that SP cells had high expression of CD13, CD29, CD44, CD46, CD49b, CD49c, CD49e, CD59, CD140a, and CD166 but low expression of CD 49d, and CD51. No evidence of expression was obtained for CD34, CD45, CD49a, CD56, CD90, CD105, CD106, CD117, CD133, CD271, or Flk-1. Upon appropriate differentiation protocols, AMC-SP cells differentiated to several cell lineages such as neuroectodermal, osteogenic, chondrogenic, and adipogenic cells. These results indicate that AMC-SP cells have multilineage potential to several cell lineages with unique immunological characteristics such as HLA I–/II– or HLA I+/II–. AMC-SP cells should be of considerable value for regenerative medicine because they do not induce acute rejection after allotransplantation, they do not cause ethical issues, and there is no limit of supply.

【 授权许可】

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