Evidence for Hepatocyte Differentiation from Embryonic Stem Cells In Vitro

Cell Transplantation

Article

Hiromitsu Nakauchi¹  Atsushi Suzuki²  Hideki Taniguchi²  Hitoshi Miyashita²  Katashi Fukao² 

[1] Department of Immunology, Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan;Department of Surgery, Institute of Clinical Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan;

关键词: Embryonic stem cell;    Embryoid bodies;    Hepatocyte;    Definitive endoderm;   

DOI  :  10.3727/000000002783985675

来源: Sage Journals

【 摘 要 】

We confirmed hepatocyte differentiation from embryonic stem (ES) cells in vitro. RT-PCR analysis revealed that a broad range of hepatic gene expression was observed in ES cells differentiated through formation of embryoid bodies (EBs) and its attachment culture. Quantitative PCR analysis revealed that hepatic gene expression related to early and late-stage liver development were enhanced through in vitro differentiation of ES cells. The presence of albumin-producing cells in the peripheral region of attached EBs was confirmed by immunocytochemical analysis. Future experiments will reveal the molecules that induce hepatocyte differentiation from ES cells in vitro. This research will provide systems for the investigation of mechanisms in liver development and establish a method of ES cell-based therapy for liver diseases.

【 授权许可】

Unknown   
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