Human Amnion-Isolated Cells Normalize Blood Glucose in Streptozotocin-Induced Diabetic Mice

Cell Transplantation

Article

Toru Aizawa¹  Masao Ota²  Ikuo Konishi³  Kiyoshi Kato³  Toshihiro Akaike⁴  Toshio Nikaido⁴  Jun Ping Wei⁴  Shigeyuki Kawa⁵  Tian Shu Zhang⁶ 

[1]Department of Aging Medicine and Geriatrics, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, Japan

[2]Department of Legal Medicine, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, Japan

[3]Department of Obstetrics and Gynecology, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, Japan

[4]Department of Organ Regeneration, Institute of Organ Transplants, Reconstructive Medicine and Tissue Engineering, 3-1-1 Asahi, Matsumoto 390-8621, Japan

[5]Second Department of Internal Medicine, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, Japan

[6]Second Department of Surgery, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, Japan

关键词: Cell transplantation;    Human;    Amnion;    Insulin;    Diabetes;   

DOI  :  10.3727/000000003108747000

来源: Sage Journals

【 摘 要 】

Whole pancreas or β-cell transplantation has opened the way for the treatment of advanced stage of diabetes mellitus. However, it is always limited by the scarcity of transplantation materials. The amniotic membrane is part of the fetal membrane and is composed of amniotic epithelium (HAE) and mesenchymal (HAM) cells that are derived from the inner cell mass in the blastocyst. Thus, HAE and HAM cells may have the potential to differentiate into various organs. The aim of our study was to assess the possibility of HAE cells differentiating into insulin-producing cells. In vitro, HAE cells stimulated with nicotinamide induced insulin mRNA in the culture cells. In vivo, HAE cells were capable of normalizing the blood glucose level of diabetic mice after several weeks of implantation into streptozotocin-induced diabetic mice. The distribution of human cells and human insulin secretion in mouse tissue studied by immunohistochemistry for anti-human-specific β-2-microglobulin and anti-human-specific insulin shows the same location in mouse tissue. These studies suggest that HAE cells have the potential to differentiate into β-cells in vivo, and hence that HAE cells have therapeutic potential for the treatment of type I diabetes mellitus.

【 授权许可】

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