Costimulation blockade by combining CTLA4Ig with anti-CD40L mAb markedly inhibits the inflammatory response of experimental autoimmune myocarditis

European Journal of Inflammation

Letters to the editor

Yu Zeng¹  Min Chen¹  Xiu-Lin Yang¹  Qing-Quan Chen¹  Xu-Chen Lin¹  Liu Qi-Cai²  Li-Hua Zhang³ 

[1] Department of Laboratory Medicine, Medical Technology and Engineering College, Fujian Medical University, Fuzhou, Fujian, PR China;Department of Laboratory Medicine, Medical Technology and Engineering College, Fujian Medical University, Fuzhou, Fujian, PR China;Department of Laboratory Medicine, the First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, PR China;Department of Laboratory Medicine, the Affiliated Union Hospital of Fujian Medical University, Fuzhou, Fujian, PR China;

关键词: anti-CD40L mAb;    autoimmune myocarditis;    costimulation blockade;    CTLA4Ig;   

DOI  :  10.1177/1721727X16686980

 received in 2016-08-27, accepted in 2016-11-29,  发布年份 2017

来源: Sage Journals

【 摘 要 】

The aim of this study was to investigate the effect of costimulation blockade with cytotoxic T-lymphocyte-associated-antigen 4-immunoglobulin (CTLA4Ig) and anti-CD40L monoclonal antibody (anti-CD40L mAb) on an experimental autoimmune myocarditis (EAM) mouse model. Characteristics of myocardial tissue were observed by hematoxylin and eosin (H&E) staining. The messenger RNA (mRNA) levels of CTLA4, CD40L, IFN-γ, and IL-4 were detected by real-time fluorescence quantitative polymerase chain reaction (RT-qPCR). Serum concentrations of IFN-γ and IL-4 were determined by ELISA. After immune intervention, the inflammatory score, mRNA levels of CTLA4 and CD40L, and IFN-γ level were decreased. Furthermore, these parameters in the combinational intervention group (blockade by CTLA4Ig and anti-CD40L mAb) were significantly decreased, compared to the single intervention group (blockade by CTLA4Ig or anti-CD40L mAb). However, after costimulation, blockade serum IL-4 levels were increased. Therefore, costimulation blockade by combination CTLA4Ig and anti-CD40L mAb could more effectively inhibit the inflammatory response of EAM than single use of CTLA4Ig or anti-CD40L mAb.

【 授权许可】

CC BY-NC   
© The Author(s) 2017

【 图 表 】

Figure 1.

Figure 2.