Impact of IL-33 on PGD2 Generation by Activated Human Cord Blood-Derived Mast Cell: Lack of Effect on Tryptase Release

European Journal of Inflammation

Original Article

D. Tripodi¹  M. Nicoletti²  T.C. Theoharides³  A. Saggini⁴  F. Conti⁵  M. Rosati⁵  P. Conti⁶  M.L. Castellani⁶  E. Tomato⁶  G. Maccauro⁷  A. Caraffa⁸  P. Antinolfi⁸  G. Potalivo⁸ 

[1] Dental School, University of Chieti-Pescara, Chieti, Italy;Department of Neurosciences and Imaging, University of Chieti, Italy;Department of Physiology and Pharmacology, Tufts University School of Medicine, New England Medical Center, Boston, MA, USA;Dermatology Department, University Tor Vergata, Rome, Italy;Gynecology Division, “Santo Spirito” Hospital, Pescara, Italy;Immunology Division, University of Chieti-Pescara, Chieti, Italy;Orthopedics Division, Catholic University of Rome, Rome, Italy;Orthopedics Division, University of Perugia, Perugia, Italy;

关键词: IL-33;    mast cells;    inflammation;    PGD2;   

DOI  :  10.1177/1721727X1201000323

 received in 2012-04-28, accepted in 2012-09-13,  发布年份 2012

来源: Sage Journals

【 摘 要 】

Mast cells are important not only in allergic reactions, but also in inflammation and are involved in a variety of responses including the immediate release of potent inflammatory mediators after activation by cross-linking of FcεRI molecules. Prostaglandin D2 (PGD2) is a major cyclooxygenase metabolite of arachidonic acid produced by mast cells and it is released following allergen challenge in allergic diseases. IL-33 is an iflammatory cytokine which is critically involved in the regulation of in vitro and in vivo cyclooxygenase production, providing a potential therapeutic target for inflammatory disorders. In this study, using human derived umbelical cord blood mast cells, we show that IL-33 (50 ng/ml), and calcium ionophore A 23187 (0.5 μg/ml), compound 48/80 (10−5 M) or anti-IgE (10 μg/ml), enhaced the production of PGD2 and this effect was inhibited by indomethacin. However, IL-33 was unable to induce tryptase release in these cells. These effects confirm the inflammatory property of IL-33 by stimulating PGD2 but not tryptase in human mast cells. The inhibitory effect of this new cytokine may have a potential therapeutic response in allergic and inflammatory diseases.

【 授权许可】

Unknown   
© 2012 SAGE Publications