Triple-color single-molecule imaging for analysis of the role of receptor oligomers in signal transduction

Biophysics and Physicobiology

Hideaki Yoshimura¹ 

[1] Department of Chemistry, School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan;

关键词: live imaging;    g protein-coupled receptor (gpcr);    signal transduction;    logic gate;    plasma membrane;   

DOI  :  10.2142/biophysico.bppb-v19.0007

来源: DOAJ

【 摘 要 】

Membrane receptors provide interfaces of various extracellular stimuli to transduce the signal into the cell. Receptors are required to possess such conflicting properties as high sensitivity and noise reduction for the cell to keep its homeostasis and appropriate responses. To understand the mechanisms by which these functions are achieved, single-molecule monitoring of the motilities of receptors and signaling molecules on the plasma membrane is one of the most direct approaches. This review article introduces several recent single-molecule imaging studies of receptors, including the author’s recent work on triple-color single-molecule imaging of G protein-coupled receptors. Based on these researches, advantages and perspectives of the single-molecule imaging approach to solving the mechanisms of receptor functions are illustrated.

【 授权许可】

Unknown