Optical analysis of AMPAR-mediated synaptic scaling in mouse hippocampus

STAR Protocols

Ege T. Kavalali¹  Lisa M. Monteggia²  Kanzo Suzuki² 

[1] Corresponding author;Department of Pharmacology and the Vanderbilt Brain Institute, Vanderbilt University, Nashville, TN 37232, USA;

关键词: Cell Biology;    Microscopy;    Neuroscience;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Immunolabeling of surface AMPA receptors (AMPARs) can be used for in vivo or ex vivo examination of synaptic scaling, a type of homeostatic plasticity. Here, we present a protocol to analyze changes in synaptic weights using immunohistochemistry for surface AMPARs coupled with optical imaging analysis. We detail immunostaining of AMPARs in mouse brain sections, followed by confocal imaging of surface AMPARs in dendritic region of hippocampal CA1. We then describe using Fiji/ImageJ and rank order plots for analyzing synaptic weight.For complete details on the use and execution of this protocol, please refer to Suzuki et al. (2021). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

【 授权许可】

Unknown