Genome-Wide Association Study of QTLs Conferring Resistance to Bacterial Leaf Streak in Rice

Plants

Suhong Bu¹  Yanyi Lin²  Xiaofang Xie³  Yinsong Liu³  Yan Zheng³  Jiazheng Yuan⁴  Libin Lu⁵  Huazhong Guan⁶  Jie Hu⁶  Weiren Wu⁶ 

[1] College of Agriculturem, South China Agricultural University, Guangzhou 510342, China;College of Arts College of Landscape Architecture, Fujian Agriculture & Forestry University, Fuzhou 350002, China;College of Life Sciences, Fujian Agriculture & Forestry University, Fuzhou 350002, China;Department of Biological and Forensic Sciences, Fayetteville State University, Fayetteville, NC 28301, USA;Fujian Academy of Agricultural Sciences, Fuzhou 350000, China;Fujian Key Laboratory of Crop Breeding by Design, Fujian Agriculture & Forestry University, Fuzhou 350002, China;

关键词: rice;    bacterial leaf streak;    disease resistance;    multi-locus GWAS;    QTN;   

DOI  :  10.3390/plants10102039

来源: DOAJ

【 摘 要 】

Bacterial leaf streak (BLS) is a devastating rice disease caused by the bacterial pathogen, Xanthomonas oryzae pv. oryzicola (Xoc), which can result in severe damage to rice production worldwide. Based on a total of 510 rice accessions, trialed in two seasons and using six different multi-locus GWAS methods (mrMLM, ISIS EM-BLASSO, pLARmEB, FASTmrMLM, FASTmrEMMA and pKWmEB), 79 quantitative trait nucleotides (QTNs) reflecting 69 QTLs for BLS resistance were identified (LOD > 3). The QTNs were distributed on all chromosomes, with the most distributed on chromosome 11, followed by chromosomes 1 and 5. Each QTN had an additive effect of 0.20 (cm) and explained, on average, 2.44% of the phenotypic variance, varying from 0.00–0.92 (cm) and from 0.00–9.86%, respectively. Twenty-five QTNs were detected by at least two methods. Among them, qnBLS11.17 was detected by as many as five methods. Most of the QTNs showed a significant interaction with their environment, but no QTNs were detected in both seasons. By defining the QTL range for each QTN according to the LD half-decay distance, a total of 848 candidate genes were found for nine top QTNs. Among them, more than 10% were annotated to be related to biotic stress resistance, and five showed a significant response to Xoc infection. Our results could facilitate the in-depth study and marker-assisted improvement of rice resistance to BLS.

【 授权许可】

Unknown