Bioengineered | |
Piperine synergistically enhances the effect of temozolomide against temozolomide-resistant human glioma cell lines | |
Somi Jeong1  Juhyun Shin1  Seunghwa Jung1  Jae-Wook Oh1  Gyun-Seok Park2  | |
[1] Konkuk Institute of Technology, Konkuk University;Konkuk University; | |
关键词: piperine; temozolomide; glioblastoma multiforme; inhibition of cell growth; apoptosis; | |
DOI : 10.1080/21655979.2020.1794100 | |
来源: DOAJ |
【 摘 要 】
Temozolomide (TMZ) is an alkylating chemotherapy agent used in the clinical treatment of glioblastoma multiforme (GBM) patients. Piperine (PIP) is a naturally occurring pungent nitrogenous substance present in the fruits of peppers. We investigated the anti-cancer efficacies of PIP alone and in combination with TMZ in GBM cellsusingparameters such as cell proliferation, cellular apoptosis,caspase-8/-9/-3 activities, cell cycle kinetics, wound-healing ability, and loss of mitochondrial membrane potential (MMP). Treatment with PIP and alow concentration of PIP-TMZ, inhibited cell growth, similar to TMZ.PIP-TMZ promoted apoptosis by activation of caspase-8/-9/-3, MMP loss, and inhibition of in vitro wound-healing motility. Reverse transcription polymerase chain reaction analysis showed significant inhibition of Cyclin-dependent kinases (CDK)4/6−cyclin D and CDK2−cyclin-E expression upon treatment with a low concentration PIP-TMZ, suggesting an S to G1 arrest. Our findings provide insight into the apoptotic potential of the combination of a low concentration of PIP-TMZ, though further in vivo study will be needed for its validation.
【 授权许可】
Unknown