Novel Aspects of Polynucleotide Phosphorylase Function in Streptomyces

Antibiotics

George H. Jones¹ 

[1] Department of Biology, Emory University, Atlanta, GA 30322, USA;

关键词: polynucleotide phosphorylase;    Streptomyces;    ribonuclease;    regulation;    promoter;    RNA decay;    polyadenylation;    (p)ppGpp;    antibiotic;   

DOI  :  10.3390/antibiotics7010025

来源: DOAJ

【 摘 要 】

Polynucleotide phosphorylase (PNPase) is a 3′–5′-exoribnuclease that is found in most bacteria and in some eukaryotic organelles. The enzyme plays a key role in RNA decay in these systems. PNPase structure and function have been studied extensively in Escherichia coli, but there are several important aspects of PNPase function in Streptomyces that differ from what is observed in E. coli and other bacterial genera. This review highlights several of those differences: (1) the organization and expression of the PNPase gene in Streptomyces; (2) the possible function of PNPase as an RNA 3′-polyribonucleotide polymerase in Streptomyces; (3) the function of PNPase as both an exoribonuclease and as an RNA 3′-polyribonucleotide polymerase in Streptomyces; (4) the function of (p)ppGpp as a PNPase effector in Streptomyces. The review concludes with a consideration of a number of unanswered questions regarding the function of Streptomyces PNPase, which can be examined experimentally.

【 授权许可】

Unknown