Differences in epitope‐specific antibodies to pertussis toxin after infection and acellular vaccinations

Clinical & Translational Immunology

Jussi Mertsola¹  Aapo Knuutila²  Alex‐Mikael Barkoff²  Qiushui He²  Kevin Markey³  Kurt Fuursted⁴  Charlotte Sværke Jørgensen⁴  Tine Dalby⁴ 

[1] Department of Pediatrics and Adolescent Medicine Turku University Hospital Turku Finland;Institute of Biomedicine University of Turku Turku Finland;National Institute for Biological Standards and Control Potters Bar UK;Statens Serum Institut Copenhagen Denmark;

关键词: ELISA;    epitope;    monoclonal antibody;    pertussis;    pertussis toxin;   

DOI  :  10.1002/cti2.1161

来源: DOAJ

【 摘 要 】

Abstract Objectives Pertussis toxin (PT) is a component of all acellular pertussis vaccines. PT must be detoxified to be included in acellular vaccines, which results in conformational changes in the functional epitopes of PTs. Therefore, induced epitope‐specific antibodies to PT may vary after vaccinations or natural infections, and this information could reveal biomarkers implicated for protection and successful immunisation. Methods Pertussis toxin epitope‐specific antibodies in sera from 152 vaccinated children and 72 serologically confirmed patients were tested with a blocking ELISA, based on monoclonal antibodies that target protective PT epitopes. Results All study groups induced considerable antibody titres to subunit 1 (S1). Of interest, S3 7E10‐specific antibodies were present in patients, but not after vaccinations (P < 0.001). The impact of glutaraldehyde treatment of PT was visible on epitope 1D7 (S1), whereas epitopes 1B7 (S1) and 10D (S1) were more preserved. Antibodies to these epitopes were higher after three primary vaccine doses than after a single booster dose. Conclusion The high amount of 7E10‐specific antibodies in patients suggests this epitope might be functionally relevant in protection. The overall characteristics of epitope‐specific antibodies are influenced by infection or vaccination background, by the used detoxification method of PT and by the amount of the toxin used in immunisation.

【 授权许可】

Unknown