Sox1a mediates the ability of the parapineal to impart habenular left-right asymmetry

eLife

Véronique Duboc¹  Stephanos Nicolaou²  Ana Faro³  Stephen W Wilson⁴  Patrick Blader⁴  Ingrid Lekk⁴ 

[1] Center for Brain Research, Medical University of Vienna, Vienna, Austria;Université Côte d'Azur, CHU, Inserm, CNRS, IRCAN, Nice, France;Centre de Biologie Intégrative (FR 3743), Centre de Biologie du Développement (UMR5547), Université de Toulouse, CNRS, Toulouse, France;Department of Cell and Developmental Biology, University College London, London, United Kingdom;

关键词: brain asymmetry;    habenulae;    pineal complex;    epithalamus;    diencephalon;    brain development;   

DOI  :  10.7554/eLife.47376

来源: DOAJ

【 摘 要 】

Left-right asymmetries in the zebrafish habenular nuclei are dependent upon the formation of the parapineal, a unilateral group of neurons that arise from the medially positioned pineal complex. In this study, we show that both the left and right habenula are competent to adopt left-type molecular character and efferent connectivity upon the presence of only a few parapineal cells. This ability to impart left-sided character is lost in parapineal cells lacking Sox1a function, despite the normal specification of the parapineal itself. Precisely timed laser ablation experiments demonstrate that the parapineal influences neurogenesis in the left habenula at early developmental stages as well as neurotransmitter phenotype and efferent connectivity during subsequent stages of habenular differentiation. These results reveal a tight coordination between the formation of the unilateral parapineal nucleus and emergence of asymmetric habenulae, ensuring that appropriate lateralised character is propagated within left and right-sided circuitry.

【 授权许可】

Unknown