Cross-Species Comparisons of Nanoparticle Interactions with Innate Immune Systems: A Methodological Review

Nanomaterials

Alessandra Prinelli¹  Eleonora Ferrari²  Craig Mayall³  Sara Michelini⁴  Manon Auguste⁵  Francesco Barbero⁶  Andi Alijagic⁷  Benjamin J. Swartzwelter⁸  Natividad Isabel Navarro Pacheco⁹  Szabolcs Hernadi^1,10  Elmer Swart^1,11 

[1] AvantiCell Science, Ltd., Ayr KA6 5HW, UK;Center for Plant Molecular Biology–ZMBP Eberhard-Karls University Tübingen, 72076 Tübingen, Germany;Department of Biology, Biotechnical Faculty, University of Liubljana, 1000 Ljubljana, Slovenia;Department of Biosciences, Paris-Lodron University Salzburg, 5020 Salzburg, Austria;Department of Earth Environment and Life Sciences, University of Genova, 16126 Genova, Italy;Institut Català de Nanosciència i Nanotecnologia (ICN2), Bellaterra, 08193 Barcelona, Spain;Institute for Biomedical Research and Innovation, National Research Council, 90146 Palermo, Italy;Institute of Biochemistry and Cell Biology, National Research Council, 80131 Napoli, Italy;Institute of Microbiology of the Czech Academy of Sciences, 142 20 Prague, Czech Republic;School of Biosciences, Cardiff University, Cardiff CF10 3AX, UK;UK Centre for Ecology and Hydrology, Wallingford OX10 8BB, UK;

关键词: environmental models;    human cells;    innate immunity;    markers;    NPs testing;   

DOI  :  10.3390/nano11061528

来源: DOAJ

【 摘 要 】

Many components of the innate immune system are evolutionarily conserved and shared across many living organisms, from plants and invertebrates to humans. Therefore, these shared features can allow the comparative study of potentially dangerous substances, such as engineered nanoparticles (NPs). However, differences of methodology and procedure between diverse species and models make comparison of innate immune responses to NPs between organisms difficult in many cases. To this aim, this review provides an overview of suitable methods and assays that can be used to measure NP immune interactions across species in a multidisciplinary approach. The first part of this review describes the main innate immune defense characteristics of the selected models that can be associated to NPs exposure. In the second part, the different modes of exposure to NPs across models (considering isolated cells or whole organisms) and the main endpoints measured are discussed. In this synergistic perspective, we provide an overview of the current state of important cross-disciplinary immunological models to study NP-immune interactions and identify future research needs. As such, this paper could be used as a methodological reference point for future nano-immunosafety studies.

【 授权许可】

Unknown