Aptamer Cocktail to Detect Multiple Species of Mycoplasma in Cell Culture

International Journal of Molecular Sciences

Zihua Zeng¹  Xiaohui Liu¹  Zhenghu Chen¹  Youli Zu¹  Quanyuan Wan¹  Yanting Liu¹ 

[1] Department of Pathology and Genomic Medicine, Houston Methodist Hospital, 6565 Fannin Street, Houston, TX 77030, USA;

关键词: DNA aptamer;    mycoplasma;    cell culture;    rapid detection;   

DOI  :  10.3390/ijms21113784

来源: DOAJ

【 摘 要 】

Mycoplasma contamination of cell line cultures is a common, yet often undetected problem in research laboratories. Many of the existing techniques to detect mycoplasma contamination of cultured cells are time-consuming, expensive, and have significant drawbacks. Here, we describe a mycoplasma detection system that is useful for detecting multiple species of mycoplasma in infected cell lines. The system contains three dye-labeled detection aptamers that can specifically bind to mycoplasma-infected cells and a dye-labeled control aptamer that minimally binds to cells. With this system, mycoplasma-contaminated cells can be detected within 30 min by using a flow cytometer, fluorescence microscope, or microplate reader. Further, this system may be used to detect mycoplasma-contaminated culture medium. This study presents an novel mycoplasma detection model that is simple, rapid, inexpensive, and sensitive.

【 授权许可】

Unknown