β3GnT8 Promotes Colorectal Cancer Cells Invasion via CD147/MMP2/Galectin3 Axis

Frontiers in Physiology

Zhi Jiang¹  Junxing Lv¹  Shiliang Wu¹  Jun Yin²  Shan Tong²  Shaohua Wei³  Huan Zhang⁴  Chunliang Liu⁵ 

[1]Department of Biochemistry and Molecular Biology, School of Medicine, Soochow University, Suzhou, China

[2]Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, China

[3]Department of General Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, China

[4]First People’s Hospital of Changshu City, Changshu Hospital Affiliated to Soochow University, Changshu, China

[5]Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China

关键词: β3GnT8;    polylactosamine;    cell invasion;    colorectal cancer;    glycosylation;   

DOI  :  10.3389/fphys.2018.00588

来源: DOAJ

【 摘 要 】

β1,3-N-acetylglucosaminyltransferase (β3GnT8) and β3GnT2 are key enzymes that catalyzes the formation of polylactosamine glycan structures by transferring GlcNAc to tetra-antennary β1-6-branched N-glycan and it also has an important effect on the progression of various types of human cancer. They have been reported to participate in tumor invasion and metastasis by regulating the expression of matrix metalloproteinases (MMPs), CD147, and polylactosamine. However, whether β3GnT8 and β3GnT2 play a role in colorectal cancer and, if so, the underlying mechanisms remain unclear. In our study, we detected the expression of β3GnT8, CD147, MMP2, and galectin3 by immunohistochemistry on 90 paraffin-embedded slices. And β3GnT8, CD147, MMP2, and galectin3 were over-expressed in colorectal cancer tissues. We found that overexpression of β3GnT8 and β3GnT2 promoted invasion of colorectal cancer cells, whereas knockdown of β3GnT8 and β3GnT2 inhibited the invasive activity. Mechanistically, β3GnT8 and β3GnT2 regulated the expression of HG-CD147 and the level of polylactosamines in colorectal cancer cells. Together, these results illustrate that the novel role and the molecular mechanism of β3GnT8 and β3GnT2 in promotion of colorectal cancer invasion. These results suggest that the potential use of β3GnT8 as a tumor target for the therapy of colorectal cancer.

【 授权许可】

Unknown