Pan-Genome Analysis Reveals Host-Specific Functional Divergences in Burkholderia gladioli

Microorganisms

Hyejung Jung¹  Hyun-Hee Lee¹  Jungwook Park¹  Young-Su Seo¹ 

[1] Department of Integrated Biological Science, Pusan National University, Busan 46241, Korea;

关键词: Burkholderia gladioli;    comparative genomics;    pan-genome;    plant pathogen;    human pathogen;   

DOI  :  10.3390/microorganisms9061123

来源: DOAJ

【 摘 要 】

Burkholderia gladioli has high versatility and adaptability to various ecological niches. Here, we constructed a pan-genome using 14 genome sequences of B. gladioli, which originate from different niches, including gladiolus, rice, humans, and nature. Functional roles of core and niche-associated genomes were investigated by pathway enrichment analyses. Consequently, we inferred the uniquely important role of niche-associated genomes in (1) selenium availability during competition with gladiolus host; (2) aromatic compound degradation in seed-borne and crude oil-accumulated environments, and (3) stress-induced DNA repair system/recombination in the cystic fibrosis-niche. We also identified the conservation of the rhizomide biosynthetic gene cluster in all the B. gladioli strains and the concentrated distribution of this cluster in human isolates. It was confirmed the absence of complete CRISPR/Cas system in both plant and human pathogenic B. gladioli and the presence of the system in B. gladioli living in nature, possibly reflecting the inverse relationship between CRISPR/Cas system and virulence.

【 授权许可】

Unknown