Genome-Wide Analysis of LncRNA in Bovine Mammary Epithelial Cell Injuries Induced by Escherichia Coli and Staphylococcus Aureus

International Journal of Molecular Sciences

Changmin Hu¹  Jinghan Yang¹  Haojun Xu¹  Changjie Lin¹  Ting Li¹  Yingyu Chen²  Xi Chen²  Aizhen Guo²  Yifan Zhu²  Zhiyu Hao² 

[1] Department of Clinical Veterinary Medicine, Faculty of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China;State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China;

关键词: mastitis;    LncRNA;    E. coli;    S. aureus;    lncRNA–miRNA–mRNA networks;   

DOI  :  10.3390/ijms22189719

来源: DOAJ

【 摘 要 】

Escherichia coli and Staphylococcus aureus are two common pathogenic microorganisms that cause mastitis in dairy cows. They can cause clinical mastitis and subclinical mastitis. In recent studies, lncRNAs have been found to play an important role in the immune responses triggered by microbial inducers. However, the actions of lncRNAs in bovine mastitis remain unclear. The purpose of this study was to investigate the effects of bovine mammary epithelial cell injuries induced by treatment with E. coli and S. aureus, and to explore the lncRNA profile on cell injuries. The lncRNA transcriptome analysis showed a total of 2597 lncRNAs. There were 2234 lncRNAs differentially expressed in the E. coli group and 2334 in the S. aureus group. Moreover, we found that the E. coli and S. aureus groups of maternal genes targeted signaling pathways with similar functions according to KEGG and GO analyses. Two lncRNA–miRNA–mRNA interaction networks were constructed in order to predict the potential molecular mechanisms of regulation in the cell injuries. We believe that this is the first report demonstrating the dysregulation of lncRNAs in cells upon E. coli and S. aureus infections, suggesting that they have the potential to become important diagnostic markers and to provide novel insights into controlling and preventing mastitis.

【 授权许可】

Unknown