Vaccination of Diffuse Large B- Cell Lymphoma Patients with Antigen-Primed Dendritic Cells

Acta Medica Iranica

Ghasem Janbabaei Mollae¹  Kamran Alimoghaddam²  Yousef Yousefzadeh³  Saeid Abediankenari³  Masoud Bahrami⁴  Maryam Ghasemi⁵ 

[1] Department of Hematology and Oncology, Mazandaran University of Medical Sciences, Sari, Iran.;Department of Hematology and Oncology, Transplantation Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.;Department of Immunology, Immonogenetic Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.;Department of Orthopedic, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.;Department of Pathology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.;

关键词: Dendritic cell;    Lymphoma;    Vaccine;   

DOI  :  

来源: DOAJ

【 摘 要 】

Dendritic cells (DCs) are professional antigen presenting cells that have a potential role in the initiating of immune responses. The cell vaccination is a new strategy in treatment of infectious diseases and cancers. In this study, we have generated monocyte-derived dendritic cells of lymphoma patient's peripheral blood mononuclear cells then; these cells were used as vaccine in lymphoma patients. We generated dendritic cell vaccine from lymphoma patient's blood monocytes with human interleukin-4, granulocyte monocyte colony stimulating factor and then, antigen-primed Dcs were administrated subcutaneously close to the inguinal lymph nodes after maturation of dendritic cells. After 7 days, we analyzed immune response in lymphoma patients with determining of LDH, Beta 2 Microglobulin, CD4+T cell percent, CD8+ Tcell percent and Tumor size before and after vaccination. Furthermore, phenotypic and functional analysis of dendritic cells was performed using anti CD83-FITC monoclonal antibodies. Before vaccination, the mean ± SD of LDH was 530.62±140.65 but after vaccination it was 459±109.45 that significantly different between experimental groups (P=0.002). In addition, the CD8+ T cells percentage significantly different between two groups (P=0.002). We concluded that the use of dendritic cell probably is one of the suitable noninvasive treatments for lymphoma patients that they have not response to chemical drugs.

【 授权许可】

Unknown