Oryza sativa cv. Nipponbare and Oryza barthii as Unexpected Tolerance and Susceptibility Sources Against Schizotetranychus oryzae (Acari: Tetranychidae) Mite Infestation

Frontiers in Plant Science

Raul Antonio Sperotto¹  Thainá Inês Lamb¹  Joséli Schwambach²  Édina Aparecida dos Reis Blasi³  Giseli Buffon³  Janete Mariza Adamski⁴  Felipe Klein Ricachenevsky⁵  Vanildo Silveira⁶  Amanda Bertolazi⁶  Mara Cristina Barbosa Lopes⁷ 

[1] Biological Sciences and Health Center, University of Taquari Valley-Univates, Lajeado, Brazil;Graduate Program in Biotechnology, University of Caxias do Sul, Caxias do Sul, Brazil;Graduate Program in Biotechnology, University of Taquari Valley-Univates, Lajeado, Brazil;Graduate Program in Botany, Federal University of Rio Grande do Sul, Porto Alegre, Brazil;Graduate Program in Molecular and Cellular Biology, Federal University of Rio Grande do Sul, Porto Alegre, Brazil;Laboratory of Biotechnology, Bioscience and Biotechnology Center, State University of Northern Rio de Janeiro Darcy Ribeiro, Campos dos Goytacazes, Brazil;Rice Research Institute (Instituto Rio-Grandense do Arroz), Cachoeirinha, Brazil;

关键词: osmotin;    proline;    protease inhibitors;    proteome;    resistance;    wild species;   

DOI  :  10.3389/fpls.2021.613568

来源: DOAJ

【 摘 要 】

Cultivated rice (Oryza sativa L.) is frequently exposed to multiple stresses, including Schizotetranychus oryzae mite infestation. Rice domestication has narrowed the genetic diversity of the species, leading to a wide susceptibility. This work aimed to analyze the response of two African rice species (Oryza barthii and Oryza glaberrima), weedy rice (O. sativa f. spontanea), and O. sativa cv. Nipponbare to S. oryzae infestation. Surprisingly, leaf damage, histochemistry, and chlorophyll concentration/fluorescence indicated that the African species present a higher level of leaf damage, increased accumulation of H2O2, and lower photosynthetic capacity when compared to O. sativa plants under infested conditions. Infestation decreased tiller number, except in Nipponbare, and caused the death of O. barthii and O. glaberrima plants during the reproductive stage. While infestation did not affect the weight of 1,000 grains in both O. sativa, the number of panicles per plant was affected only in O. sativa f. spontanea, and the percentage of full seeds per panicle and seed length were increased only in Nipponbare. Using proteomic analysis, we identified 195 differentially abundant proteins when comparing susceptible (O. barthii) and tolerant (Nipponbare) plants under control and infested conditions. O. barthii presents a less abundant antioxidant arsenal and is unable to modulate proteins involved in general metabolism and energy production under infested condition. Nipponbare presents high abundance of detoxification-related proteins, general metabolic processes, and energy production, suggesting that the primary metabolism is maintained more active compared to O. barthii under infested condition. Also, under infested conditions, Nipponbare presents higher levels of proline and a greater abundance of defense-related proteins, such as osmotin, ricin B-like lectin, and protease inhibitors (PIs). These differentially abundant proteins can be used as biotechnological tools in breeding programs aiming at increased tolerance to mite infestation.

【 授权许可】

Unknown