Analysis of Cadmium Root Retention for Two Contrasting Rice Accessions Suggests an Important Role for OsHMA2

Plants

Giampiero Valè¹  Giorgio Lucchini²  Moez Maghrebi²  GianAttilio Sacchi²  FabioFrancesco Nocito²  Gianpiero Vigani³  Elena Baldoni⁴ 

[1] Centro di ricerca per la Cerealicoltura e le Colture Industriali (CREA-CI), 13100 Vercelli, Italy;Dipartimento di Scienze Agrarie e Ambientali—Produzione, Territorio, Agroenergia (DISAA), Università degli Studi di Milano, 20133 Milano, Italy;Dipartimento di Scienze della Vita e Biologia dei Sistemi, Università degli Studi di Torino, 10135 Torino, Italy;Istituto di Biologia e Biotecnologia Agraria (IBBA), Consiglio Nazionale delle Ricerche (CNR), 20133 Milano, Italy;

关键词: Oryza sativa L.;    cadmium;    zinc;    cadmium translocation;    cadmium root retention;   

DOI  :  10.3390/plants10040806

来源: DOAJ

【 摘 要 】

Two rice accessions, Capataz and Beirao, contrasting for cadmium (Cd) tolerance and root retention, were exposed to a broad range of Cd concentrations (0.01, 0.1, and 1 μM) and analyzed for their potential capacity to chelate, compartmentalize, and translocate Cd to gain information about the relative contribution of these processes in determining the different pathways of Cd distribution along the plants. In Capataz, Cd root retention increased with the external Cd concentration, while in Beirao it resulted independent of Cd availability and significantly higher than in Capataz at the lowest Cd concentrations analyzed. Analysis of thiol accumulation in the roots revealed that the different amounts of these compounds in Capataz and Beirao, as well as the expression levels of genes involved in phytochelatin biosynthesis and direct Cd sequestration into the vacuoles of the root cells, were not related to the capacity of the accessions to trap the metal into the roots. Interestingly, the relative transcript abundance of OsHMA2, a gene controlling root-to-shoot Cd/Zn translocation, was not influenced by Cd exposure in Capataz and progressively increased in Beirao with the external Cd concentration, suggesting that activity of the OsHMA2 transporter may differentially limit root-to-shoot Cd/Zn translocation in Capataz and Beirao.

【 授权许可】

Unknown