Coordinated recruitment of Spir actin nucleators and myosin V motors to Rab11 vesicle membranes

eLife

Patrick England¹  Petra Schwille¹  Thomas Weidemann²  Olena Pylypenko³  Gilles Malherbe³  Florian Chardon³  Bruno Goud³  Bruno Baron⁴  Margaret A Titus⁴  Martin Kollmar⁵  Anne Houdusse⁶  Janine Tittel⁶  Eugen Kerkhoff⁶  Andreas Till Grasskamp⁷  Carina Ida Luise Michel⁷  Annette Samol-Wolf⁷  Tobias Welz⁷  Sabine Weiss⁷  Alistair Hume⁸ 

[1] CNRS, UMR 3528, Paris, France;Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, United States;Institut Curie, PSL Research University, CNRS, UMR 144, F-75005, Paris, France;Institut Pasteur, Biophysics of Macromolecules and their Interactions, Paris, France;Max Planck Institute for Biophysical Chemistry, Göttingen, Germany;Max Planck Institute of Biochemistry, Martinsried, Germany;University Hospital Regensburg, Regensburg, Germany;University of Nottingham, Nottingham, United Kingdom;

关键词: actin nucleation;    actin motor proteins;    vesicle transport;    myosin V;    Rab11;    Spire;   

DOI  :  10.7554/eLife.17523

来源: DOAJ

【 摘 要 】

There is growing evidence for a coupling of actin assembly and myosin motor activity in cells. However, mechanisms for recruitment of actin nucleators and motors on specific membrane compartments remain unclear. Here we report how Spir actin nucleators and myosin V motors coordinate their specific membrane recruitment. The myosin V globular tail domain (MyoV-GTD) interacts directly with an evolutionarily conserved Spir sequence motif. We determined crystal structures of MyoVa-GTD bound either to the Spir-2 motif or to Rab11 and show that a Spir-2:MyoVa:Rab11 complex can form. The ternary complex architecture explains how Rab11 vesicles support coordinated F-actin nucleation and myosin force generation for vesicle transport and tethering. New insights are also provided into how myosin activation can be coupled with the generation of actin tracks. Since MyoV binds several Rab GTPases, synchronized nucleator and motor targeting could provide a common mechanism to control force generation and motility in different cellular processes.

【 授权许可】

Unknown