Lysosomes appear as the auto-fluorescent vacuoles in Dictyostelium discoideum cells

Pteridines

Saito Yuya¹  Omata Takako A.²  Uchiyama Saburo³  Hatakeyama Ikuo⁴ 

[1] ;Clinical Laboratory, University Hospital, Dokkyo Medical University, Mibu, Tochigi, Japan;Laboratory of Biology, Course of Science Education, Graduate School of Education, Iwate University, Ueda 3-18-33, Morioka 020-8550, Japan;Laboratory of Biology, Studies in Science Education, Graduate School of Education, Iwate University, Morioka, Japan;

关键词: auto-fluorescence;    chloroquine;    dictyostelium;    lysosome;    nbd-pz;   

DOI  :  10.1515/pterid-2015-0014

来源: DOAJ

【 摘 要 】

Dictyostelium discoideum cells contain auto-fluorescent vacuoles. To determine the identity of these vacuoles, the fluorescent dye 4-nitro-7-(1-piperazinyl)-2,1,3-benzoxadiazole (NBD-PZ) was used to stain the lysosomes in D. discoideum cells. Neither the auto-fluorescent vacuoles nor lysosomes were observed in D. discoideum cells immediately after they arose from spores or in stationary phase cells. However, both the auto-fluorescent vacuoles and lysosomes were visible in cells that had entered growth phase. Auto-fluorescent vacuoles and lysosomes were also observed in stationary phase cells incubated with chloroquine. When the cells were allowed to phagocytose BioParticles Fluorescent Bacteria (orange fluorescence) for 1 h, orange phagosomes and blue auto-fluorescent vacuoles were observed as independent moieties. However, after an additional 2 h of incubation, we observed vacuoles with mixed fluorescence (orange and blue) in the cells, suggestive of secondary lysosomes. These results suggest that the auto-fluorescent vacuoles in D. discoideum cells are lysosomes.

【 授权许可】

Unknown