Surface Modification of Porous Polyethylene Implants with an Albumin-Based Nanocarrier-Release System

Biomedicines

Sven Becker¹  Juergen Brieger²  Sebastian Strieth²  Niklas Hoormann²  Jonas Eckrich²  Martin Heller²  Toni Anusic³  Erik Kersten⁴  Keti Piradashvili⁴  Frederik R. Wurm⁴ 

[1] Department of Otorhinolaryngology, Head and Neck Surgery, University of Tübingen, Elfriede-Aulhorn-Str. 5, 72076 Tübingen, Germany;Department of Otorhinolaryngology, University Medical Center Mainz, Langenbeckstrasse 1, 55131 Mainz, Germany;Institute of Medical Biostatistics, Epidemiology and Informatics (IMBEI), University Medical Center, Obere Zahlbacher Str. 69, 55131 Mainz, Germany;Max Planck Institute for Polymer Research (MPIP), Ackermannweg 10, 55128 Mainz, Germany;

关键词: porous polyethylene;    biomaterial;    material science;    albumin nanocarriers;    tissue engineering;    release kinetics;   

DOI  :  10.3390/biomedicines9101485

来源: DOAJ

【 摘 要 】

Background: Porous polyethylene (PPE) implants are used for the reconstruction of tissue defects but have a risk of rejection in case of insufficient ingrowth into the host tissue. Various growth factors can promote implant ingrowth, yet a long-term gradient is a prerequisite for the mediation of these effects. As modification of the implant surface with nanocarriers may facilitate a long-term gradient by sustained factor release, implants modified with crosslinked albumin nanocarriers were evaluated in vivo. Methods: Nanocarriers from murine serum albumin (MSA) were prepared by an inverse miniemulsion technique encapsulating either a low- or high-molar mass fluorescent cargo. PPE implants were subsequently coated with these nanocarriers. In control cohorts, the implant was coated with the homologue non-encapsulated cargo substance by dip coating. Implants were consequently analyzed in vivo using repetitive fluorescence microscopy utilizing the dorsal skinfold chamber in mice for ten days post implantation. Results: Implant-modification with MSA nanocarriers significantly prolonged the presence of the encapsulated small molecules while macromolecules were detectable during the investigated timeframe regardless of the form of application. Conclusions: Surface modification of PPE implants with MSA nanocarriers results in the alternation of release kinetics especially when small molecular substances are used and therefore allows a prolonged factor release for the promotion of implant integration.

【 授权许可】

Unknown