Macrophage Accumulation and Angiogenesis in Epicardial Adipose Tissue in Cardiac Patients with or without Chronic Heart Failure

Applied Sciences

RalucaE. Haliga¹  RaduA. Sascau¹  Cristian Statescu¹  AdinaPricope Veselin²  Veronica Mocanu²  Gabriela Ghiga³  VictorV. Costan⁴  Doina Butcovan⁵  DanielV. Timofte⁶  Radu Danila⁶  BogdanM. Ciuntu⁶ 

[1] Department of Internal Medicine, University of Medicine and Pharmacy, 16, Universitatii Street, 700115 Iasi, Romania;Department of Morpho-Functional Sciences II, University of Medicine and Pharmacy, 16, Universitatii Street, 70011 Iasi, Romania;Department of Mother and Child Medicine, University of Medicine and Pharmacy, 16, Universitatii Street, 700115 Iasi, Romania;Department of Oral and Maxillofacial Surgery, University of Medicine and Pharmacy, 16, Universitatii Street, 700115 Iasi, Romania;Department of Pathology, Institute of Cardiovascular Diseases, 50, Carol I Avenue, 700503 Iasi, Romania;Department of Surgery, University of Medicine and Pharmacy, 16, Universitatii Street, 700115 Iasi, Romania;

关键词: epicardial adipose tissue;    coronary artery disease;    chronic heart failure;    right atrial appendages;    coronary artery bypass graft;   

DOI  :  10.3390/app10175871

来源: DOAJ

【 摘 要 】

Routinely measuring epicardial fat had become a novel tool for cardiovascular risk stratification. Structural changes in epicardial adipose tissue (EAT), including fat thickness, inflammation, and angiogenesis, have been described in coronary artery disease (CAD) patients. We proposed to measure EAT thickness and characterize inflammatory infiltrate and angiogenesis in epicardial adipose tissue in CAD patients with and without chronic heart failure (CHF), established by cardiac dysfunction on echocardiography (left ventricular ejection fraction, LVEF ≤ 50%) and symptoms of heart failure (New York Heart Association (NYHA) functional class II or III).The study included 15 patients with CAD (demonstrated by coronary angiography),, who underwent right atrial appendages (RAA) excision during coronary artery bypass graft (CABG). The study was performed by histopathological, immunohistochemical (IHC), and morphometrical analysis. EAT thickness was assessed by using morphometry applied on routine histological stains. Inflammatory cell infiltration and angiogenesis were investigated immunohistochemically by using antibodies against CD68 and CD34 markers. Diminished EAT thickness in the CAD patients with CHF was associated with increased macrophage infiltration and reduced angiogenesis of the EAT as compared to CAD patients without CHF. In conclusion, the present study on epicardial fat samples of the RAA suggested that high expression of CD68 appeared to be associated with severe deterioration of heart function in CAD patients who underwent myocardial revascularization consisting of CABG.

【 授权许可】

Unknown