Feasibility of Parylene Coating for Planar Electroporation Copper Electrodes

Medžiagotyra

Audrius GRAINYS¹  Sonata TOLVAIŠIENĖ²  Vitalij NOVICKIJ³  Jurij Novickij³  Svetlana MARKOVSKAJA⁴  Aleksandr TABAŠNIKOV⁵  Stewart SMITH⁵ 

[1] ;Department of Electrical Engineering, Vilnius Gediminas Technical University, Naugarduko g. 41, LT-03227 Vilnius, Lithuania;Institute of High Magnetic Fields, Vilnius Gediminas Technical University, Naugarduko g. 41, LT-03227 Vilnius, Lithuania;Laboratory of Mycology, Nature Research Centre, Žaliųjų ežerų g. 49, LT-08406 Vilnius, Lithuania;Scottish Microelectronics Centre, University of Edinburgh, King’s Building, EH9 3JF Edinburgh, United Kingdom;

关键词: microelectrodes, pulsed electric field, biological cells;   

DOI  :  10.5755/j01.ms.23.2.14953

来源: DOAJ

【 摘 要 】

This paper is focused on the feasibility study of parylene as a biocompatible coating for planar electroporation microelectrodes. The planar parallel and the circular interdigitated electrodes are applied in the analysis. The electrodes feature 100 μm width with a 300 μm gap between anode and cathode. The parylene coating thickness was varied in the 250 nm – 2 μm range. The resultant electric field distribution evaluation has been performed using the finite element method. The electrodes have been applied in electroporation experiments with Saprolegnia parasitica. For reference the additional experiments using conventional electroporation cuvette (1 mm gap) have been performed. It has been determined that the parylene coating with hydrophobic properties has limited applicability for the passivation of the planar electroporation electrodes.

DOI: http://dx.doi.org/10.5755/j01.ms.23.2.14953

【 授权许可】

Unknown