Electrochemical Sensing of Idarubicin—DNA Interaction Using Electropolymerized Azure B and Methylene Blue Mediation

Chemosensors

Yurii Kuzin¹  Gennady Evtugyn¹  Anastasia Goida¹  Anna Porfireva¹  Tibor Hianik²  Vladimir Evtugyn³ 

[1] A.M. Butlerov’ Chemistry Institute, Kazan Federal University, 18 Kremlevskaya Street, 420008 Kazan, Russia;Department of Nuclear Physics and Biophysics, Comenius University, Mlynska dolina F1, 842 48 Bratislava, Slovakia;Interdisciplinary Center of Analytical Microscopy, Kazan Federal University, 18 Kremlevskaya Street, 420008 Kazan, Russia;

关键词: idarubicin;    Azure B;    electropolymerization;    DNA sensor;    voltammetry;    electrochemical impedance spectroscopy;   

DOI  :  10.3390/chemosensors10010033

来源: DOAJ

【 摘 要 】

A highly sensitive electrochemical DNA sensor for detection of the chemotherapeutic drug idarubicin mediated by Methylene blue (MB) has been developed. DNA from fish sperm has been immobilized at the electropolymerized layers of Azure B. The incorporation of MB into the DNA layers substantially increased the sensor sensitivity. The concentration range for idarubicin determination by cyclic voltammetry was from 1 fM to 0.1 nM, with a limit of detection (LOD) of 0.3 fM. Electrochemical impedance spectroscopy (EIS) in the presence of a redox probe ([Fe(CN)₆]^3−/4−) allowed for the widening of a linear range of idarubicin detection from 1 fM to 100 nM, retaining LOD 0.3 fM. The DNA sensor has been tested in various real and artificial biological fluids with good recovery ranging between 90–110%. The sensor has been successfully used for impedimetric idarubicin detection in medical preparation Zavedos^®. The developed DNA biosensor could be useful for the control of the level of idarubicin during cancer therapy as well as for pharmacokinetics studies.

【 授权许可】

Unknown