Plasma Proteomic Profile of Patients with Tick-Borne Encephalitis and Co-Infections

International Journal of Molecular Sciences

Sinemyiz Atalay¹  Agnieszka Gęgotek¹  Elżbieta Skrzydlewska¹  Monika Groth²  Piotr Czupryna²  Anna Moniuszko-Malinowska²  Sławomir Pancewicz²  Justyna Dunaj²  Piotr Radziwon³ 

[1] Department of Analytical Chemistry, Medical University of Bialystok, Mickiewicza 2D, 15-222 Bialystok, Poland;Department of Infectious Diseases and Neuroinfections, Medical University of Bialystok, Zurawia 14, 15-540 Bialystok, Poland;Regional Centre for Transfusion Medicine, M. Sklodowskiej-Curie 23, 15-950 Bialystok, Poland;

关键词: tick-borne encephalitis;    plasma;    proteomic profile;    protein adducts;    co-infections;    Lyme disease;   

DOI  :  10.3390/ijms23084374

来源: DOAJ

【 摘 要 】

Despite the increasing number of patients suffering from tick-borne encephalitis (TBE), Lyme disease, and their co-infection, the mechanisms of the development of these diseases and their effects on the human body are still unknown. Therefore, the aim of this study was to evaluate the changes in the proteomic profile of human plasma induced by the development of TBE and to compare it with changes in TBE patients co-infected with other tick-borne pathogens. The results obtained by proteomic analysis using a nanoLC-Q Exactive HF mass spectrometer showed that the most highly elevated groups of proteins in the plasma of TBE patients with co-infection were involved in the pro-inflammatory response and protein degradation, while the antioxidant proteins and factors responsible for protein biosynthesis were mainly downregulated. These results were accompanied by enhanced GSH- and 4-HNE-protein adducts formation, observed in TBE and co-infected patients at a higher level than in the case of patients with only TBE. In conclusion, the differences in the proteomic profiles between patients with TBE and co-infected patients indicate that these diseases are significantly diverse and, consequently, require different treatment, which is particularly important for further research, including the development of novel diagnostics tools.

【 授权许可】

Unknown