High-Fat Diets with Differential Fatty Acids Induce Obesity and Perturb Gut Microbiota in Honey Bee

International Journal of Molecular Sciences

Hao Zheng¹  Zhaopeng Zhong¹  Xiangyin Chen¹  Xiaofei Wang¹  Weimin Lin¹  Ziyun Hong¹  Xiaosong Hu¹  Xiaohuan Mu¹ 

[1] College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 510642, China;

关键词: gut microbiota;    dietary fat;    fat accumulation;    lipid metabolism;    honey bee;   

DOI  :  10.3390/ijms22020834

来源: DOAJ

【 摘 要 】

HFD (high-fat diet) induces obesity and metabolic disorders,which is associated with the alteration in gut microbiota profiles. However, the underlying molecular mechanisms of the processes are poorly understood. In this study, we used the simple model organism honey bee to explore how different amounts and types of dietary fats affect the host metabolism and the gut microbiota. Excess dietary fat, especially palm oil, elicited higher weight gain, lower survival rates, hyperglycemic, and fat accumulation in honey bees. However, microbiota-free honey bees reared on high-fat diets did not significantly change their phenotypes. Different fatty acid compositions in palm and soybean oil altered the lipid profiles of the honey bee body. Remarkably, dietary fats regulated lipid metabolism and immune-related gene expression at the transcriptional level. Gene set enrichment analysis showed that biological processes, including transcription factors, insulin secretion, and Toll and Imd signaling pathways, were significantly different in the gut of bees on different dietary fats. Moreover, a high-fat diet increased the relative abundance of Gilliamella, while the level of Bartonella was significantly decreased in palm oil groups. This study establishes a novel honey bee model of studying the crosstalk between dietary fat, gut microbiota, and host metabolism.

【 授权许可】

Unknown