Directional Changes in the Intestinal Bacterial Community in Black Soldier Fly (Hermetia illucens) Larvae

Animals

Jing Zhang¹  Zhihao Zhou²  Shen Zhou²  Qiang Xiong²  Xinfu Li² 

[1] College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, China;College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211800, China;

关键词: black soldier fly;    16S rRNA sequencing;    Lysinibacillus sphaericus;   

DOI  :  10.3390/ani11123475

来源: DOAJ

【 摘 要 】

Black soldier fly (BSF) larvae, Hermetia illucens (Diptera: Stratiomyidae) have emerged as an efficient system for the bioconversion of organic waste. Intestinal microorganisms are involved in several insect functions, including the development, nutrition, and physiology of the host. In order to transform the intestinal bacterial community of BSF directionally, six different potential functional strains (Lysinibacillus sphaericus, Proteus mirabilis, Citrobacter freundii, Pseudocitrobacter faecalis, Pseudocitrobacter anthropi, and Enterococcus faecalis) were added to aseptic food waste, and aseptic food waste was used without inoculants as a blank control to evaluate the changes in the intestinal microbiota of BSF under artificial intervention conditions. These six strains (which were isolated from the larval intestinal tract in selective media and then identified and screened) may be considered responsible for the functional characteristics of larvae. The results imply that the increase in the abundance of Lysinibacillus in the experimental group that was exposed to Lysinibacillus sphaericus was significantly different to the other groups (p < 0.05). The results revealed that it is feasible to transform the intestinal microbiota of BSF directionally; there are differences in the proliferation of different strains in the intestine of BSF.

【 授权许可】

Unknown