Spinal Cord Molecular and Cellular Changes Induced by Adenoviral Vector- and Cell-Mediated Triple Gene Therapy after Severe Contusion

Frontiers in Pharmacology

Andrei A. Izmailov¹  Tatyana V. Povysheva¹  Farid V. Bashirov¹  Filip O. Fadeev¹  Yuri A. Chelyshev¹  Ravil R. Garifulin¹  Mikhail E. Sokolov¹  Igor A. Lavrov³  Boris S. Naroditsky⁴  Denis Y. Logunov⁴  Ilnur I. Salafutdinov⁵  Rustem R. Islamov⁶ 

[1] Department of Biology, Kazan State Medical University, Kazan, Russia;Department of Neurologic Surgery, Mayo Clinic, Rochester, MN, United States;Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, United States;Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia;Institute of Fundamental Medicine and Biology, Kazan Federal (Volga Region) University, Kazan, Russia;Kazan Scientific Center, Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, Kazan, Russia;

关键词: spinal cord injury;    glial cells;    gene therapy;    human umbilical cord blood mononuclear cell;    vascular endothelial growth factor;    glial cell-derived neurotrophic factor;   

DOI  :  10.3389/fphar.2017.00813

来源: DOAJ

【 摘 要 】

The gene therapy has been successful in treatment of spinal cord injury (SCI) in several animal models, although it still remains unavailable for clinical practice. Surprisingly, regardless the fact that multiple reports showed motor recovery with gene therapy, little is known about molecular and cellular changes in the post-traumatic spinal cord following viral vector- or cell-mediated gene therapy. In this study we evaluated the therapeutic efficacy and changes in spinal cord after treatment with the genes encoding vascular endothelial growth factor (VEGF), glial cell-derived neurotrophic factor (GDNF), angiogenin (ANG), and neuronal cell adhesion molecule (NCAM) applied using both approaches. Therapeutic genes were used for viral vector- and cell-mediated gene therapy in two combinations: (1) VEGF+GDNF+NCAM and (2) VEGF+ANG+NCAM. For direct gene therapy adenoviral vectors based on serotype 5 (Ad5) were injected intrathecally and for cell-mediated gene delivery human umbilical cord blood mononuclear cells (UCB-MC) were simultaneously transduced with three Ad5 vectors and injected intrathecally 4 h after the SCI. The efficacy of both treatments was confirmed by improvement in behavioral (BBB) test. Molecular and cellular changes following post-traumatic recovery were evaluated with immunofluorescent staining using antibodies against the functional markers of motorneurons (Hsp27, synaptophysin, PSD95), astrocytes (GFAP, vimentin), oligodendrocytes (Olig2, NG2, Cx47) and microglial cells (Iba1). Our results suggest that both approaches with intrathecal delivery of therapeutic genes may support functional recovery of post-traumatic spinal cord via lowering the stress (down regulation of Hsp25) and enhancing the synaptic plasticity (up regulation of PSD95 and synaptophysin), supporting oligodendrocyte proliferation (up regulation of NG2) and myelination (up regulation of Olig2 and Cx47), modulating astrogliosis by reducing number of astrocytes (down regulation of GFAP and vimetin) and microglial cells (down regulation of Iba1).

【 授权许可】

Unknown